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Molecule Parameter List for PLC

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

PLC participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	0	0	2	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning	77	Network	Shared_Object_Ajay_Bhalla_2004_PKM_MKP3_Tuning,  PKC,  PLA2,   PLCbeta,  Ras,  Gq,   MAPK,  EGFR,  Sos,   PLC_g,  CaMKII,  CaM,   PP1,  PP2B,  PKA,   AC,  MKP3,  PKM
This model is based on Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80. This is the feedforward model with MPK3 from figure 8a.

PLC acting as a Molecule in  Ajay_Bhalla_2004_PKM_MKP3_Tuning Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
PLC	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	PLCbeta Pathway No. : 332	0.8	1.5	No
Total PLC = 0.8 uM see Ryu et al JBC 262 (26) pp 12511 1987

PLC acting as a Substrate in a reaction in  Ajay_Bhalla_2004_PKM_MKP3_Tuning Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	Act-PLC-Ca	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	PLCbeta Pathway No. : 332	3 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 0.3333(uM)	-	Substrate Ca PLC Product PLC-Ca
	Affinity for Ca = 1uM without AlF, 0.1 with: from Smrcka et al science 251 pp 804-807 1991 so [Ca].kf = kb so kb/kf = 1 * 6e5 = 1/1.66e-6 11 June 1996: Raised affinity to 5e-6 to maintain balance. See notes.
2	PLC-bind-Gq	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	PLCbeta Pathway No. : 332	2.52 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 0.3968(uM)	-	Substrate G*GTP PLC Product PLC-Gq
	this binding does not produce active PLC. This step was needed to implement the described (Smrcka et al) increase in affinity for Ca by PLC once Gq was bound. The kinetics are the same as the binding step for Ca-PLC to Gq. June 1996: Changed the kf to 4.2e-5 to 4.2e-6 to preserve balance around the reactions.