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Molecule Parameter List for Rec-Gq

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

Rec-Gq participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	0	0	2	1

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning	77	Network	Shared_Object_Ajay_Bhalla_2004_PKM_MKP3_Tuning,  PKC,  PLA2,   PLCbeta,  Ras,  Gq,   MAPK,  EGFR,  Sos,   PLC_g,  CaMKII,  CaM,   PP1,  PP2B,  PKA,   AC,  MKP3,  PKM
This model is based on Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80. This is the feedforward model with MPK3 from figure 8a.

Rec-Gq acting as a Molecule in  Ajay_Bhalla_2004_PKM_MKP3_Tuning Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
Rec-Gq	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	Gq Pathway No. : 334	0	1.5	No
Fraction of Rec-Gq is 44% of rec, from Fay et al. Since this is not the same receptor, this value is a bit doubtful. Still, we adjust the rate consts in Rec-bind-Gq to match.

Rec-Gq acting as a Substrate in a reaction in  Ajay_Bhalla_2004_PKM_MKP3_Tuning Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	Glu-bind-Rec-Gq	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	Gq Pathway No. : 334	16.8003 (uM^-1 s^-1)	0.1 (s^-1)	Kd(bf) = 0.006(uM)	-	Substrate Glu Rec-Gq Product Rec-Glu-Gq
	From Fay et al kb3 = kb = 1.06e-3 which is rather slow. k+1 = kf = 2.8e7 /M/sec= 4.67e-5/sec use 5e-5. However, the Kd from Martin et al may be more appropriate, as this is Glu not the system from Fay. kf = 2.8e-5, kb = 10 Let us compromise. since we have the Fay model, keep kf = k+1 = 2.8e-5. But kb (k-3) is .01 * k-1 from Fay. Scaling by .01, kb = .01 * 10 = 0.1
2	Antag-bind-Rec-G q	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	Gq Pathway No. : 334	60.0003 (uM^-1 s^-1)	0.01 (s^-1)	Kd(bf) = 0.0002(uM)	-	Substrate Rec-Gq mGluRAntag Product Blocked-rec-Gq
	The rate consts give a total binding affinity of only

Rec-Gq acting as a Product in a reaction in  Ajay_Bhalla_2004_PKM_MKP3_Tuning Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
Rec-bind-Gq	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	Gq Pathway No. : 334	0.6 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 1.6667(uM)	-	Substrate G-GDP mGluR Product Rec-Gq
Lets try out the same kinetics as the Rec-Glu-bind-Gq This is much too forward. We know that the steady-state amount of Rec-Gq should be 40% of the total amount of receptor. This is for a different receptor, still we can try to match the value. kf = 1e-6 and kb = 1 give 0.333:0.8 which is pretty close.