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Molecule Parameter List for CaMKII-thr286

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

CaMKII-thr286 participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	1	0	3	1	1	1

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning	77	Network	Shared_Object_Ajay_Bhalla_2004_PKM_MKP3_Tuning,  PKC,  PLA2,   PLCbeta,  Ras,  Gq,   MAPK,  EGFR,  Sos,   PLC_g,  CaMKII,  CaM,   PP1,  PP2B,  PKA,   AC,  MKP3,  PKM
This model is based on Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80. This is the feedforward model with MPK3 from figure 8a.

CaMKII-thr286 acting as a Molecule in  Ajay_Bhalla_2004_PKM_MKP3_Tuning Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
CaMKII-thr286	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	CaMKII Pathway No. : 339	0	1.5	No
I am not sure if we need to endow this one with a lot of enzs. It is likely to be a short-lived intermediate, since it will be phosphorylated further as soon as the CAM falls off.

CaMKII-thr286 acting as a Summed Molecule in  Ajay_Bhalla_2004_PKM_MKP3_Tuning Network

Accession Name	Pathway Name	Target	Input
Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	CaMKII Pathway No. : 339	tot_autonomous_CaMKII	CaMKII-thr286 CaMKII***

CaMKII-thr286 acting as a Substrate for an Enzyme in  Ajay_Bhalla_2004_PKM_MKP3_Tuning Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	tot_CaM_CaMKII  / CaM_act_305	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	CaMKII Pathway No. : 339	113.599	6	4	explicit E-S complex	Substrate CaMKII-thr286 Product CaMKII***
	Rates from autocamtide phosphorylation, from Hanson and Schulman JBC 267:24 17216-17224 1992. Jan 1 1998: Speed up 12x to match fig 5.
2	tot_autonomous_ CaMKII  / auton_305	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	CaMKII Pathway No. : 339	175.006	6	4	explicit E-S complex	Substrate CaMKII-thr286 Product CaMKII***
	See Hanson and Schulman again, for afterburst rates of phosph.
3	PP1-active  / Deph_thr286b	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	Shared_Object_ Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Pathway No. : 329	5.09911	0.35	4	explicit E-S complex	Substrate CaMKII-thr286 Product CaMKII

CaMKII-thr286 acting as a Product of an Enzyme in  Ajay_Bhalla_2004_PKM_MKP3_Tuning Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
PP1-active  / Deph-thr305	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	Shared_Object_ Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Pathway No. : 329	5.09911	0.35	4	explicit E-S complex	Substrate CaMKII*** Product CaMKII-thr286

CaMKII-thr286 acting as a Substrate in a reaction in  Ajay_Bhalla_2004_PKM_MKP3_Tuning Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
CaMK-thr286-bind -CaM	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	CaMKII Pathway No. : 339	1000.17 (uM^-1 s^-1)	0.1 (s^-1)	Kd(bf) = 0.0001(uM)	-	Substrate CaM-Ca4 CaMKII-thr286 Product CaMKII-thr286*-C aM
Affinity is up 1000X. Time to release is about 20 sec, so the kb is OK at 0.1 This makes Kf around 1.6666e-3

CaMKII-thr286 acting as a Product in a reaction in  Ajay_Bhalla_2004_PKM_MKP3_Tuning Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
basal-activity	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	CaMKII Pathway No. : 339	0.003 (s^-1)	0 (s^-1)	-	-	Substrate CaMKII Product CaMKII-thr286
This reaction represents one of the big unknowns in CaMK-II biochemistry: what maintains the basal level of phosphorylation on thr 286 ? See Hanson and Schulman Ann Rev Biochem 1992 61:559-601, specially pg 580, for review. I have not been able to find any compelling mechanism in the literature, but fortunately the level of basal activity is well documented.