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Molecule Parameter List for CaNAB-Ca4

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*CaNAB-Ca4* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	1	0	0	3	1

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning	77	Network	Shared_Object_Ajay_Bhalla_2004_PKM_MKP3_Tuning, PKC, PLA2, PLCbeta, Ras, Gq, MAPK, EGFR, Sos, PLC_g, CaMKII, CaM, PP1, PP2B, PKA, AC, MKP3, PKM
This model is based on Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80. This is the feedforward model with MPK3 from figure 8a.

CaNAB-Ca4 acting as a Molecule in Ajay_Bhalla_2004_PKM_MKP3_Tuning Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
CaNAB-Ca4	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	Shared_Object_ Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Pathway No. : 329	0	1.5	No

CaNAB-Ca4 acting as an Enzyme in Ajay_Bhalla_2004_PKM_MKP3_Tuning Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
CaNAB-Ca4 / dephosph_ inhib1_noCaM	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	Shared_Object_ Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Pathway No. : 329	4.97076	0.034	4	explicit E-S complex	Substrate I1* Product I1
The rates here are so slow I do not know if we should even bother with this enz reacn. These numbers are from Liu and Storm. Other refs suggest that the Km stays the same but the Vmax goes to 10% of the CaM stim levels. Prev: k1=2.2e-9, k2 = 0.0052, k3 = 0.0013 New : k1=5.7e-8, k2=.136, k3=.034

CaNAB-Ca4 acting as a Substrate in a reaction in Ajay_Bhalla_2004_PKM_MKP3_Tuning Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	CaM-Ca2-bind-CaN AB	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	PP2B Pathway No. : 342	0.24 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 4.1666(uM)	-	Substrate CaM-TR2-Ca2 CaNAB-Ca4 Product CaMCa2-CANAB
	Disabled. See notes for PP2B7.g
2	CaMCa3-bind-CaNA B	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	PP2B Pathway No. : 342	2.238 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 0.4468(uM)	-	Substrate CaM-Ca3 CaNAB-Ca4 Product CaMCa3-CaNAB
3	CaMCa4-bind-CaNA B	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	PP2B Pathway No. : 342	600.003 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 0.0017(uM)	-	Substrate CaM-Ca4 CaNAB-Ca4 Product CaMCa4-CaNAB

CaNAB-Ca4 acting as a Product in a reaction in Ajay_Bhalla_2004_PKM_MKP3_Tuning Network

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
Ca-bind-CaNAB-Ca 2	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	PP2B Pathway No. : 342	3.6 (uM^-2 s^-1)	1 (s^-1)	Kd(af) = 0.527(uM)	-	Substrate Ca Ca CaNAB-Ca2 Product CaNAB-Ca4
This process is probably much more complicated and involves CaM. However, as I can't find detailed info I am bundling this into a single step. Based on Steemer and Klee pg 6863, the Kact is 0.5 uM. kf/kb = 1/(0.5 * 6e5)^2 = 1.11e-11

Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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