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Molecule Parameter List for AC2*

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

AC2* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	1	0	1	2	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning	77	Network	Shared_Object_Ajay_Bhalla_2004_PKM_MKP3_Tuning, PKC, PLA2, PLCbeta, Ras, Gq, MAPK, EGFR, Sos, PLC_g, CaMKII, CaM, PP1, PP2B, PKA, AC, MKP3, PKM
This model is based on Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80. This is the feedforward model with MPK3 from figure 8a.

AC2* acting as a Molecule in Ajay_Bhalla_2004_PKM_MKP3_Tuning Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
AC2*	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	AC Pathway No. : 344	0	1.5	No
This version is activated by Gs and by a betagamma and phosphorylation.

AC2* acting as an Enzyme in Ajay_Bhalla_2004_PKM_MKP3_Tuning Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
AC2* / kenz	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	AC Pathway No. : 344	20.1153	7	4	explicit E-S complex	Substrate ATP Product cAMP
Reduced Km to match expt data for basal activation of AC2 by PKC. Now k1 = 2.9e-6, k2 = 72, k3 = 18

AC2* acting as a Product of an Enzyme in Ajay_Bhalla_2004_PKM_MKP3_Tuning Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
PKC-active / phosph-AC2	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	Shared_Object_ Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Pathway No. : 329	33.3332	4	4	explicit E-S complex	Substrate AC2 Product AC2*
Phorbol esters have little effect on AC1 or on the Gs-stimulation of AC2. So in this model we are only dealing with the increase in basal activation of AC2 induced by PKC k1 = 1.66e-6 k2 = 16 k3 =4

AC2* acting as a Substrate in a reaction in Ajay_Bhalla_2004_PKM_MKP3_Tuning Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	dephosph-AC2	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	AC Pathway No. : 344	0.1 (s^-1)	0 (s^-1)	-	-	Substrate AC2* Product AC2
	Random rate.
2	Gs-bind-AC2*	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	AC Pathway No. : 344	833.283 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 0.0012(uM)	-	Substrate AC2* Gs-alpha Product AC2*-Gs
	kb/kf = 1.2 nM so kf = kb/720 = 1.3888 kb.*

Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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