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Molecule Parameter List for AMP

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

AMP participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	0	4	0	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning	77	Network	Shared_Object_Ajay_Bhalla_2004_PKM_MKP3_Tuning,  PKC,  PLA2,   PLCbeta,  Ras,  Gq,   MAPK,  EGFR,  Sos,   PLC_g,  CaMKII,  CaM,   PP1,  PP2B,  PKA,   AC,  MKP3,  PKM
This model is based on Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80. This is the feedforward model with MPK3 from figure 8a.

AMP acting as a Molecule in  Ajay_Bhalla_2004_PKM_MKP3_Tuning Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
AMP	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	AC Pathway No. : 344	325493	2.5E-6	No

AMP acting as a Product of an Enzyme in  Ajay_Bhalla_2004_PKM_MKP3_Tuning Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	cAMP-PDE  / PDE	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	AC Pathway No. : 344	19.8413	10	4	explicit E-S complex	Substrate cAMP Product AMP
	Best rates are from Conti et al Biochem 34 7979-7987 1995. Though these are for the Sertoli cell form, it looks like they carry nicely into alternatively spliced brain form. See Sette et al JBC 269:28 18271-18274 Km ~2 uM, Vmax est ~ 10 umol/min/mg for pure form. Brain protein is 93 kD but this was 67. So k3 ~10, k2 ~40, k1 ~4.2e-6
2	cAMP-PDE*  / PDE*	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	AC Pathway No. : 344	19.8413	20	4	explicit E-S complex	Substrate cAMP Product AMP
	This form has about twice the activity of the unphosphorylated form. See Sette et al JBC 269:28 18271-18274 1994. We'll ignore cGMP effects for now.
3	PDE1  / PDE1	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	AC Pathway No. : 344	39.7006	1.667	4.0012	explicit E-S complex	Substrate cAMP Product AMP
	Rate is 1/6 of the CaM stim form. We'll just reduce all lf k1, k2, k3 so that the Vmax goes down 1/6.
4	CaM.PDE1  / CaM.PDE1	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	AC Pathway No. : 344	39.6825	10	4	explicit E-S complex	Substrate cAMP Product AMP
	Max activity ~10umol/min/mg in presence of lots of CaM. Affinity is low, 40 uM. k3 = 10, k2 = 40, k1 = (50/40) / 6e5.