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Molecule Parameter List for MAPKK-ser | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by  color. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| MAPKK-ser participated as | Molecule | Sum total of | Enzyme | Substrate of an enzyme | Product of an enzyme | Substrate in Reaction | Product in Reaction |
| No. of occurrences | 1 | 0 | 0 | 2 | 2 | 0 | 0 |
Accession and Pathway Details |
| Accession Name | Accession No. | Accession Type | Pathway Link |
| 3d_fold_model | 8 | Network | Shared_Object_3d_fold_model, PKC, MAPK, PLA2, Ras |
| This model is an annotated version of the synaptic signaling network. The primary reference is Bhalla US and Iyengar R. Science (1999) 283(5400):381-7 but several of the model pathways have been updated Bhalla US, Ram PT, Iyengar R. Science. 2002 Aug 9;297(5583):1018-23. | |||
MAPKK-ser acting as a Molecule in 3d_fold_model Network
| Name | Accession Name | Pathway Name | Initial Conc. (uM) | Volume (fL) | Buffered | |
| MAPKK-ser | 3d_fold_model Accession No. : 8 | MAPK Pathway No. : 56 | 0 | 1000 | No | |
| Intermediately phophorylated, assumed inactive, form of MAPKK | ||||||
MAPKK-ser acting as a Substrate for an Enzyme in 3d_fold_model Network
| Enzyme Molecule / Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | |
| 1 | Raf-GTP-Ras* / Raf-GTP-Ras*.2 | 3d_fold_model Accession No. : 8 | MAPK Pathway No. : 56 | 0.159091 | 0.105 | 4 | explicit E-S complex | Substrate MAPKK-ser Product MAPKK* |
| Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6 | ||||||||
| 2 | PPhosphatase2A / MAPKK-deph-ser | 3d_fold_model Accession No. : 8 | 3d_fold_model Pathway No. : 54 | 15.6566 | 6 | 4.16667 | explicit E-S complex | Substrate MAPKK-ser Product MAPKK |
MAPKK-ser acting as a Product of an Enzyme in 3d_fold_model Network
| Enzyme Molecule / Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | |
| 1 | Raf-GTP-Ras* / Raf-GTP-Ras*.1 | 3d_fold_model Accession No. : 8 | MAPK Pathway No. : 56 | 0.159091 | 0.105 | 4 | explicit E-S complex | Substrate MAPKK Product MAPKK-ser |
| Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6 | ||||||||
| 2 | PPhosphatase2A / MAPKK-deph | 3d_fold_model Accession No. : 8 | 3d_fold_model Pathway No. : 54 | 15.6566 | 6 | 4.16667 | explicit E-S complex | Substrate MAPKK* Product MAPKK-ser |
| See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms. | ||||||||