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Molecule Parameter List for temp-PIP2

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

temp-PIP2 participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	0	0	2	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
MAPK_MKP1_ oscillation	9	Network	Shared_Object_MAPK_MKP1_oscillation,  PKC,  MAPK,   PLA2,  Ras
This model relates to figure 5 in Bhalla US, Iyengar R. Chaos (2001) 11(1):221-226. It includes the model used for figures 2-4 and also has MKP-1 induction by MAPK activity in the synapse. PP2A is set to 0.16 uM and MKP synthesis is varied from 5x to 40 x basal to get a range of interesting behaviours.

temp-PIP2 acting as a Molecule in  MAPK_MKP1_oscillation Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
temp-PIP2	MAPK_MKP1_ oscillation Accession No. : 9	Shared_Object_ MAPK_MKP1_ oscillation Pathway No. : 59	2.5	1000	Yes
This isn't explicitly present in the M&L model, but is obviously needed. I assume its conc is fixed at 1uM for now, which is a bit high. PLA2 is stim 7x by PIP2 @ 0.5 uM (Leslie and Channon BBA 1045:261(1990) Leslie and Channon say PIP2 is present at 0.1 - 0.2mol% range in membs, which comes to 50 nM. Ref is Majerus et al Cell 37 pp 701-703 1984 Lets use a lower level of 30 nM, same ref....

temp-PIP2 acting as a Substrate in a reaction in  MAPK_MKP1_oscillation Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	PIP2-PLA2-act	MAPK_MKP1_ oscillation Accession No. : 9	PLA2 Pathway No. : 62	0.0012 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 416.6667(uM)	-	Substrate PLA2-cytosolic temp-PIP2 Product PIP2-PLA2*
2	PIP2-Ca-PLA2-act	MAPK_MKP1_ oscillation Accession No. : 9	PLA2 Pathway No. : 62	0.012 (uM^-1 s^-1)	0.1 (s^-1)	Kd(bf) = 8.3333(uM)	-	Substrate PLA2-Ca* temp-PIP2 Product PIP2-Ca-PLA2*