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Molecule Parameter List for PLA2-cytosolic

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*PLA2-cytosolic* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	1	0	2	1

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
MAPK_MKP1_ oscillation	9	Network	Shared_Object_MAPK_MKP1_oscillation, PKC, MAPK, PLA2, Ras
This model relates to figure 5 in Bhalla US, Iyengar R. Chaos (2001) 11(1):221-226. It includes the model used for figures 2-4 and also has MKP-1 induction by MAPK activity in the synapse. PP2A is set to 0.16 uM and MKP synthesis is varied from 5x to 40 x basal to get a range of interesting behaviours.

PLA2-cytosolic acting as a Molecule in MAPK_MKP1_oscillation Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
PLA2-cytosolic	MAPK_MKP1_ oscillation Accession No. : 9	PLA2 Pathway No. : 62	0.4	1000	No
Calculated cytosolic was 20 nm from Wijkander and Sundler However, Leslie and Channon use about 400 nM. Need to confirm, but this is the value I use here. Another recalc of W&S gives 1uM

PLA2-cytosolic acting as a Substrate for an Enzyme in MAPK_MKP1_oscillation Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
MAPK* / MAPK*	MAPK_MKP1_ oscillation Accession No. : 9	Shared_Object_ MAPK_MKP1_ oscillation Pathway No. : 59	25.641	20	4	explicit E-S complex	Substrate PLA2-cytosolic Product PLA2*
Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5

PLA2-cytosolic acting as a Substrate in a reaction in MAPK_MKP1_oscillation Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	PLA2-Ca-act	MAPK_MKP1_ oscillation Accession No. : 9	PLA2 Pathway No. : 62	1 (uM^-1 s^-1)	0.1 (s^-1)	Kd(bf) = 0.1(uM)	-	Substrate Ca PLA2-cytosolic Product PLA2-Ca*
	Leslie and Channon BBA 1045 (1990) 261-270 fig6 pp267.
2	PIP2-PLA2-act	MAPK_MKP1_ oscillation Accession No. : 9	PLA2 Pathway No. : 62	0.0012 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 416.6667(uM)	-	Substrate PLA2-cytosolic temp-PIP2 Product PIP2-PLA2*

PLA2-cytosolic acting as a Product in a reaction in MAPK_MKP1_oscillation Network

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
dephosphorylate- PLA2*	MAPK_MKP1_ oscillation Accession No. : 9	PLA2 Pathway No. : 62	0.17 (s^-1)	0 (s^-1)	-	-	Substrate PLA2* Product PLA2-cytosolic

Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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