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Enzyme List for pathway Ras (Pathway Number 58) in Accession 3d_fold_model (Accession Number 8) |
Entries are grouped according to Pathway Number and they are alternately color coded using  and  color.Further ordering can be done according to header.   arrow indicates that ordering is done according to ascending or descending order. |
| Enzyme Molecule / Enzyme Activity | Pathway Name / Pathway No. | Km (uM) | kcat (s^-1) | Ratio (k2/k3) | Enzyme Type | Reagents | ||
| 1 | GEF* / GEF*-act-ras | Ras Pathway No. 58 | 0.505051 | 0.02 | 4 | explicit E-S complex | Substrate: GDP-Ras Product : GTP-Ras | |
| Kinetics same as GEF-bg-act-ras | ||||||||
| 2 | GAP / GAP-inact-ras | Ras Pathway No. 58 | 1.0104 | 10 | 100 | explicit E-S complex | Substrate: GTP-Ras Product : GDP-Ras | |
| From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) | ||||||||