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Reaction List for pathway PKA (Pathway Number 240) in Accession AMPAR_traff_model0 (Accession Number 59) |
Entries are grouped according to Pathway Number and they are alternately color coded using  and  color.
Further ordering can be done to the table header.   indicates that ordering is done according to ascending or descending order.Keq is calculated only for first order reactions. Kd is calculated only for second order reactions. [nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules] |
| Reaction Name | Pathway Name / Pathway No. | Kf | Kb | Kd | tau | Reagents | |
| 1 | 1 | PKA Pathway No. 240 | 75.0006 (uM^-1 s^-1) | 110 (s^-1) | Kd(bf) = 1.4667(uM) | - | Substrate: R2C2-cAMP2 cAMP Products: R2C2-cAMP3 |
| 2 | 2 | PKA Pathway No. 240 | 75.0006 (uM^-1 s^-1) | 32.5 (s^-1) | Kd(bf) = 0.4333(uM) | - | Substrate: cAMP R2C2-cAMP3 Products: R2C2-cAMP4 |
| 3 | Release-C1 | PKA Pathway No. 240 | 60 (s^-1) | 17.9998 (uM^-1 s^-1) | Kd(cb) = 0.3(uM) | - | Substrate: R2C2-cAMP4 Products: PKA-active R2C-cAMP4 | This has to be fast, as the activation of PKA by cAMP is also fast. kf was 10 |
| 4 | Release-C2 | PKA Pathway No. 240 | 60 (s^-1) | 17.9998 (uM^-1 s^-1) | Kd(cb) = 0.3(uM) | - | Substrate: R2C-cAMP4 Products: PKA-active R2-cAMP4 |
| 5 | inhib-PKA | PKA Pathway No. 240 | 59.9994 (uM^-1 s^-1) | 1 (s^-1) | Kd(bf) = 0.0167(uM) | - | Substrate: PKA-active PKA-inhibitor Products: inhibited-PKA | This has to be set to zero for matching the expts in vitro. In vivo we need to consider the inhibition though. kf = 1e-5 kb = 1 |
| 6 | 1 | PKA Pathway No. 240 | 54 (uM^-1 s^-1) | 33 (s^-1) | Kd(bf) = 0.6111(uM) | - | Substrate: R2C2 cAMP Products: R2C2-cAMP | Hasler et al FASEB J 6:2734-2741 1992 say Kd =1e-7M for type II, 5.6e-8 M for type I. Take mean which comes to 2e-13 #/cell Smith et al PNAS USA 78:3 1591-1595 1981 have better data. First kf/kb=2.1e7/M = 3.5e-5 (#/cell). Ogreid and Doskeland Febs Lett 129:2 287-292 1981 have figs suggesting time course of complete assoc is < 1 min. |
| 7 | 2 | PKA Pathway No. 240 | 54 (uM^-1 s^-1) | 33 (s^-1) | Kd(bf) = 0.6111(uM) | - | Substrate: R2C2-cAMP cAMP Products: R2C2-cAMP2 | For now let us set this to the same Km (1e-7M) as site B. This gives kf/kb = .7e-7M * 1e6 / (6e5^2) : 1/(6e5^2) = 2e-13:2.77e-12 Smith et al have better values. They say that this is cooperative, so the consts are now kf/kb =8.3e-4 |