Enter a Search String |
| Special character and space not allowed in the query term. Search string should be at least 2 characters long. |
Enzyme List for pathway PLA2 (Pathway Number 208) in Accession MAPK_network_2003 (Accession Number 50) |
Entries are grouped according to Pathway Number and they are alternately color coded using  and  color.Further ordering can be done according to header.   arrow indicates that ordering is done according to ascending or descending order. |
| Enzyme Molecule / Enzyme Activity | Pathway Name / Pathway No. | Km (uM) | kcat (s^-1) | Ratio (k2/k3) | Enzyme Type | Reagents | ||
| 1 | PLA2-Ca* / kenz | PLA2 Pathway No. 208 | 20 | 5.4 | 4 | explicit E-S complex | Substrate: APC Product : AA | |
| 10 x raise oct22 12 x oct 24, set k2 = 4 * k3 | ||||||||
| 2 | PIP2-PLA2* / kenz | PLA2 Pathway No. 208 | 20 | 11.04 | 4 | explicit E-S complex | Substrate: APC Product : AA | |
| 10 X raise oct 22 12 X further raise oct 24 to allow for correct conc of enzyme | ||||||||
| 3 | PIP2-Ca-PLA2* / kenz | PLA2 Pathway No. 208 | 20 | 36 | 4 | explicit E-S complex | Substrate: APC Product : AA | |
| 10 x raise oct 22 12 x and rescale for k2 = 4 * k3 convention oct 24 Increase further to get the match to expt, which was spoilt due to large accumulation of PLA2 in the enzyme complexed forms. Lets raise k3, leaving the others at k1 = 1.5e-5 and k2 = 144 since they are large already. | ||||||||
| 4 | DAG-Ca-PLA2* / kenz | PLA2 Pathway No. 208 | 20 | 60 | 4 | explicit E-S complex | Substrate: APC Product : AA | |
| 10 X raise oct 22 12 X raise oct 24 + conversion to k2 =4 * k3 | ||||||||
| 5 | PLA2*-Ca / kenz | PLA2 Pathway No. 208 | 20 | 120 | 4 | explicit E-S complex | Substrate: APC Product : AA | |
| This form should be 3 to 6 times as fast as the Ca-only form. I have scaled by 4x which seems to give a 5x rise. 10x raise Oct 22 12 x oct 24, changed k2 = 4 * k3 | ||||||||