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Molecule Parameter List for AA

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

AA participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	0	5	4	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
MAPK_network_ 2003	50	Network	Shared_Object_MAPK_network_2003, PKC, PLA2, PLCbeta, Gq, MAPK, Ras, EGFR, Sos, PLC_g, CaMKII, CaM, PP1, PP2B, PKA, AC
This is a network model of many pathways present at the neuronal synapse. The network has properties of temporal tuning as well as steady-state computational properties. In its default form the network is bistable.Bhalla US Biophys J. 2004 Aug;87(2):745-53

AA acting as a Molecule in MAPK_network_2003 Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
AA	MAPK_network_ 2003 Accession No. : 50	Shared_Object_ MAPK_network_ 2003 Pathway No. : 206	0	1000	No

AA acting as a Product of an Enzyme in MAPK_network_2003 Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	PLA2-Ca* / kenz	MAPK_network_ 2003 Accession No. : 50	PLA2 Pathway No. : 208	20	5.4	4	explicit E-S complex	Substrate APC Product AA
	10 x raise oct22 12 x oct 24, set k2 = 4 k3*
2	PIP2-PLA2* / kenz	MAPK_network_ 2003 Accession No. : 50	PLA2 Pathway No. : 208	20	11.04	4	explicit E-S complex	Substrate APC Product AA
	10 X raise oct 22 12 X further raise oct 24 to allow for correct conc of enzyme
3	PIP2-Ca-PLA2* / kenz	MAPK_network_ 2003 Accession No. : 50	PLA2 Pathway No. : 208	20	36	4	explicit E-S complex	Substrate APC Product AA
	10 x raise oct 22 12 x and rescale for k2 = 4 * k3 convention oct 24 Increase further to get the match to expt, which was spoilt due to large accumulation of PLA2 in the enzyme complexed forms. Lets raise k3, leaving the others at k1 = 1.5e-5 and k2 = 144 since they are large already.
4	DAG-Ca-PLA2* / kenz	MAPK_network_ 2003 Accession No. : 50	PLA2 Pathway No. : 208	20	60	4	explicit E-S complex	Substrate APC Product AA
	10 X raise oct 22 12 X raise oct 24 + conversion to k2 =4 k3*
5	PLA2*-Ca / kenz	MAPK_network_ 2003 Accession No. : 50	PLA2 Pathway No. : 208	20	120	4	explicit E-S complex	Substrate APC Product AA
	This form should be 3 to 6 times as fast as the Ca-only form. I have scaled by 4x which seems to give a 5x rise. 10x raise Oct 22 12 x oct 24, changed k2 = 4 k3*

AA acting as a Substrate in a reaction in MAPK_network_2003 Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	PKC-act-by-Ca-AA	MAPK_network_ 2003 Accession No. : 50	PKC Pathway No. : 207	0.0012 (uM^-1 s^-1)	0.1 (s^-1)	Kd(bf) = 83.3333(uM)	-	Substrate AA PKC-Ca Product PKC-Ca-AA*
	Schaechter and Benowitz We have to increase Kf for conc scaling Changed kf to 2e-9 on Sept 19, 94. This gives better match.
2	PKC-act-by-AA	MAPK_network_ 2003 Accession No. : 50	PKC Pathway No. : 207	0.0001 (uM^-1 s^-1)	0.1 (s^-1)	Kd(bf) = 833.3333(uM)	-	Substrate AA PKC-cytosolic Product PKC-AA*
	Raise kf from 1.667e-10 to 2e-10 to get better match to data.
3	PKC-n-DAG-AA	MAPK_network_ 2003 Accession No. : 50	PKC Pathway No. : 207	0.018 (uM^-1 s^-1)	2 (s^-1)	Kd(bf) = 111.1111(uM)	-	Substrate AA PKC-DAG Product PKC-DAG-AA
	Reduced kf to 0.66X to match Shinomura et al data. Initial: kf = 3.3333e-9 New: 2e-9 Newer: 2e-8 kb was 0.2 now 2.
4	Degrade-AA	MAPK_network_ 2003 Accession No. : 50	PLA2 Pathway No. : 208	0.4 (s^-1)	0 (s^-1)	-	-	Substrate AA Product APC
	I need to check if the AA degradation pathway really leads back to APC. Anyway, it is a convenient buffered pool to dump it back into. For the purposes of the full model we use a rate of degradation of 0.2/sec Raised decay to 0.4 : see PLA35.g notes for Feb17

Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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