Enter a Search String |
| Special character and space not allowed in the query term. Search string should be at least 2 characters long. |
Enzyme List for pathway Ras (Pathway Number 391) in Accession Ajay_bhalla_2007_ReacDiff1_1e-12 (Accession Number 81) |
Entries are grouped according to Pathway Number and they are alternately color coded using  and  color.Further ordering can be done according to header.   arrow indicates that ordering is done according to ascending or descending order. |
| Enzyme Molecule / Enzyme Activity | Pathway Name / Pathway No. | Km (uM) | kcat (s^-1) | Ratio (k2/k3) | Enzyme Type | Reagents | ||
| 1 | GEF* / GEF*-act-ras | Ras Pathway No. 391 | 0.505057 | 0.02 | 4 | explicit E-S complex | Substrate: GDP-Ras Product : GTP-Ras | |
| Kinetics same as GEF-bg-act-ras | ||||||||
| 2 | GAP / GAP-inact-ras | Ras Pathway No. 391 | 1.0104 | 10 | 4 | explicit E-S complex | Substrate: GTP-Ras Product : GDP-Ras | |
| From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting. | ||||||||
| 3 | CaM-GEF / CaM-GEF-act-ras | Ras Pathway No. 391 | 0.505057 | 0.2 | 4 | explicit E-S complex | Substrate: GDP-Ras Product : GTP-Ras | |
| Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input. | ||||||||
| 4 | inact-GEF / activity | Ras Pathway No. 391 | 10.1015 | 0.02 | 4 | explicit E-S complex | Substrate: GDP-Ras Product : GTP-Ras | |