| | Molecule Name/
Site Name | Km (uM) | kcat (1/s) | Ratio
(k2/k3) | Enzyme Type | Substrate | Product |
| 1 |
Enzyme Activity:
E1*_deactiv
Enzyme Molecule:
P1 | 0.0600001 | 0.1 | 5 | explicit E-S complex | E1*
| E1
|
| | kp1+ =6*10^8 M-1 min-1, kp1- =30 min-1, kpcat,1 =6 min-1 form Asthagiri and Lauffenburger 2001. |
| 2 |
Enzyme Activity:
E1_activ
Enzyme Molecule:
E0* | 0.0600001 | 0.1 | 5 | explicit E-S complex | E1
| E1*
|
| | k1+ =6*10^8 M-1 min-1, k1- =30 min-1, kcat,1 =6 min-1 and km caluculated to 0.06 from Asthagiri and Lauffenburger 2001. |
| 3 |
Enzyme Activity:
E2*_deactiv
Enzyme Molecule:
P2 | 0.0600001 | 0.1 | 5 | explicit E-S complex | E2*
| E2
|
| | kp2+ =6*10^8 M-1 min-1, kp2- = 30 min-1, kpcat,2 = 6 min-1 from Asthagiri and Lauffenburger 2001. |
| 4 |
Enzyme Activity:
E2_activ
Enzyme Molecule:
E1* | 0.0600001 | 0.1 | 5 | explicit E-S complex | E2
| E2*
|
| | k2+ =6*10^8 M-1 min-1, k2- =30 min-1, kcat,2 =6 min-1 from Asthagire and Lauffenburger 2001 |
| 5 |
Enzyme Activity:
E3*_deactiv
Enzyme Molecule:
P3 | 0.0600001 | 0.1 | 5 | explicit E-S complex | E3*
| E3
|
| | kp3+ =6*10^8 M-1 min-1, kp3- =30 min-1, kpcat,3 =6 min-1 from Asthagiri and Lauffenburger 2001. |
| 6 |
Enzyme Activity:
E3_activ
Enzyme Molecule:
E2* | 0.0600001 | 0.1 | 5 | explicit E-S complex | E3
| E3*
|
| | k3+ =6*10^8 M-1 min-1, k3- =30 min-1, kcat,3 =6 min-1 from Asthagiri and Lauffenburger 2001. |
| 7 |
Enzyme Activity:
E4*_deactiv
Enzyme Molecule:
P4 | 0.0600001 | 0.1 | 5 | explicit E-S complex | E4*
| E4
|
| | kp4+ = 6*10^8 M-1 min-1, kp4- =30 min-1, kpcat,4 =6 min-1 from Asthagiri and Lauffenburger 2001. |
| 8 |
Enzyme Activity:
E4*_enz_
targeted_fb
Enzyme Molecule:
E4* | 0.0600001 | 0.1 | 5 | explicit E-S complex | E2*
| E2-
|
| | Implementation of enzyme feedback induces adaptation of e2* and signals downstream of the target signal, but not those signals upstream of the target e0* and e1*. k5+ =6*10^8 M-1 min-1, k5- =30 min-1, kcat,5 =6 min-1 from Asthagiri and Lauffenburger 2001. |
| 9 |
Enzyme Activity:
E4_activ
Enzyme Molecule:
E3* | 0.0600001 | 0.1 | 5 | explicit E-S complex | E4
| E4*
|
| | k4+ = 6*10^8 M-1 min-1, k4- =30 min-1, kcat,4 =6 min-1 from Asthagiri and Lauffenburger 2001. |
| 10 |
Enzyme Activity:
E5*_deactiv
Enzyme Molecule:
P5 | 0.0600001 | 0.1 | 5 | explicit E-S complex | E5*
| E5
|
| | kp5+ = 6*10^8 M-1 min-1, kp5- = 30 min-1, kpcat,5 = 6 min-1 from Asthagiri and Lauffenburger 2001. |
| 11 |
Enzyme Activity:
E5_activ
Enzyme Molecule:
E4* | 0.0600001 | 0.1 | 5 | explicit E-S complex | E5
| E5*
|
| | k5+ =6*10^8 M-1 min-1, k5- =30 min-1, kcat,5 =6 min-1 from Asthagiri and Lauffenburger 2001. |
