| | Molecule Name/
Site Name | Km (uM) | kcat (1/s) | Ratio
(k2/k3) | Enzyme Type | Substrate | Product |
| 1 |
Enzyme Activity:
phosph-AC2
Enzyme Molecule:
PKC-active | 33.3337 | 4 | 4 | explicit E-S complex | AC2
| AC2*
|
| | Phorbol esters have little effect on AC1 or on the Gs-stimulation of AC2. So in this model we are only dealing with the increase in basal activation of AC2 induced by PKC k1 = 1.66e-6 k2 = 16 k3 =4 |
| 2 |
Enzyme Activity:
PKC-phosph-neuro
granin
Enzyme Molecule:
PKC-active | 28.6273 | 0.58 | 4.03448 | explicit E-S complex | neurogranin
| neurogranin*
|
| | Rates from Huang et al ABB 305:2 570-580 1993 |
| 3 |
Enzyme Activity:
PKC-phosph-neuro
granin_
PSD
Enzyme Molecule:
PKC-active | 28.6273 | 0.58 | 4.03448 | explicit E-S complex | neurogranin_PSD
| neurogranin*_
PSD
|
| | Rates from Huang et al ABB 305:2 570-580 1993 |
| 4 |
Enzyme Activity:
PKC-phosph-ng-Ca
M
Enzyme Molecule:
PKC-active | 28.5956 | 0.35 | 4 | explicit E-S complex | neurogranin-CaM
| CaM
neurogranin*
|
| | Rates are 60% those of PKC-phosph-neurogranin. See Huang et al ABB 305:2 570-580 1993 |
| 5 |
Enzyme Activity:
PKC-phosph-ng-Ca
M_
PSD
Enzyme Molecule:
PKC-active | 28.5956 | 0.35 | 4 | explicit E-S complex |
neurogranin-CaM_
PSD
| CaM-PSD
neurogranin*_
PSD
|
| | Rates are 60% those of PKC-phosph-neurogranin. See Huang et al ABB 305:2 570-580 1993 |
