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Enzyme List for pathway LacI (Pathway Number 300)

 Molecule Name/
Site Name 		Km (uM) kcat (1/s)Ratio
(k2/k3)		Enzyme TypeSubstrate Product
1 Enzyme Activity:
Lac_basal_
transcription

Enzyme Molecule:
Lac_operator		0.00062164Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S		Nucleotides
lac_mRNA1
  Basal transcription of the Lac gene. Rates from Fig2 Garcia & Elowitz et al. (2004) PNAS 101:10955-60
2 Enzyme Activity:
Lac_
transcription

Enzyme Molecule:
Lac_operator_
1st_bound		0.00060.00054Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S		Nucleotides
lac_mRNA1
  Transcription of the lac gene with only one of its promoter sites activated. Rates from Box 1 Elowitz, M. et al. (2000) N Nature 403: 335-338
3 Enzyme Activity:
Lac_
transcription1

Enzyme Molecule:
Lac_operator_
2nd_bound		0.00060.00054Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S		Nucleotides
lac_mRNA1
  Transcription of the Lac gene with both its promoter sites activated. Rates from Box 1 Elowitz, M. et al. (2000) Nature 403: 335-338
4 Enzyme Activity:
lac_translation

Enzyme Molecule:
lac_mRNA1		0.00060.1674Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S		Amino_Acids
Lac-1
  Translation of the lac mRNA. Rates from Box 1 Elowitz, M. et al (2000) Nature 403: 335-338
Pathway DetailMolecule ListEnzyme ListReaction List