| | Molecule Name/
Site Name | Km (uM) | kcat (1/s) | Ratio
(k2/k3) | Enzyme Type | Substrate | Product |
| 1 |
Enzyme Activity:
Lac_active_
transcription
Enzyme Molecule:
Lac_operator_
activator_bound | 0.0005556 | 20 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | Nucleotides
| lac_mRNA
|
| | Lac transcription from the activator bound Lac gene. kcat is taken from Garcia & Elowitz et al. (2004) PNAS 101: 10955-60 |
| 2 |
Enzyme Activity:
Lac_basal_
transcription
Enzyme Molecule:
Lac_operator | 0.000555607 | 216 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | Nucleotides
| lac_mRNA
|
| | Basal transcription of the Lac gene. Rates from Fig 2 Garcia and Elowitz et al.(2004) PNAS 101: 10955-60 |
| 3 |
Enzyme Activity:
Lac_
transcription
Enzyme Molecule:
Lac_operator_
1st_bound | 0.0005556 | 0.0005 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | Nucleotides
| lac_mRNA
|
| | Transcription of the Lac gene with only one of its promoter sites activated. Rates from Box 1 Elowitz, M. et al. (2000) N Nature 403: 335-338 |
| 4 |
Enzyme Activity:
Lac_
transcription1
Enzyme Molecule:
Lac_operator_
2nd_bound | 0.0005556 | 0.0005 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | Nucleotides
| lac_mRNA
|
| | Transcription of the Lac gene with both its promoter sites activated. Rates from Box 1 Elowitz, M. et al. (2000) Nature 403: 335-338 |
| 5 |
Enzyme Activity:
lac_translation
Enzyme Molecule:
lac_mRNA | 0.000555595 | 0.167 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | Amino_Acids
| Lac-1
|
| | Translation of the lac mRNA. Rates from Box 1 Elowitz, M. et al (2000) Nature 403: 335-338 |
