| | Molecule Name/
Site Name | Km (uM) | kcat (1/s) | Ratio
(k2/k3) | Enzyme Type | Substrate | Product |
| 1 |
Enzyme Activity:
LuxI_basal_
transcription
Enzyme Molecule:
LuxI_operator | 0.000555607 | 216 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | Nucleotides
| luxI_mRNA
|
| | Basal transcription of the Lux I gene. Garcia-Ojalvo, J. et al. (2004) PNAS USA 101(30):10955-10960 As described for components of the Repressilator in Box 1 Elowitz, M. et al. (2000) Nature 403:335-338 |
| 2 |
Enzyme Activity:
LuxI_
transcription
Enzyme Molecule:
LuxI_operator_
1st_bound | 0.0005556 | 0.0005 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | Nucleotides
| luxI_mRNA
|
| | Transcription of the LuxI gene with only one of its promoter sites activated. Garcia-Ojalvo, J. et al (2004) PNAS USA 101(30):10955-10960 As described for components of the Repressilator in Box 1 Elowitz, M. et al. (2000) Nature 403:335-338 |
| 3 |
Enzyme Activity:
LuxI_
transcription1
Enzyme Molecule:
LuxI_operator_
2nd_bound | 0.0005556 | 0.0005 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | Nucleotides
| luxI_mRNA
|
| 4 |
Enzyme Activity:
luxI_
translation
Enzyme Molecule:
luxI_mRNA | 0.000555595 | 0.167 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | Amino_Acids
| LuxI
|
| | Rates from Box 1 Elowitz et al (2003) Nature 403:335-338 |
