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Enzyme List for pathway MAPK (Pathway Number 914)

 Molecule Name/
Site Name 		Km (uM) kcat (1/s)Ratio
(k2/k3)		Enzyme TypeSubstrate Product
1 Enzyme Activity:
MAPKKthr

Enzyme Molecule:
MAPKK*		0.04630.34explicit E-S complexMAPK-tyr
MAPK*
  Rate consts same as for MAPKKtyr.
2 Enzyme Activity:
MAPKKtyr

Enzyme Molecule:
MAPKK*		0.04630.34explicit E-S complexMAPK
MAPK-tyr
  The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
3 Enzyme Activity:
Raf*-GTP-Ras.1

Enzyme Molecule:
Raf*-GTP-Ras		0.15910.34explicit E-S complexMAPKK
MAPKK-ser
  Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
4 Enzyme Activity:
Raf*-GTP-Ras.2

Enzyme Molecule:
Raf*-GTP-Ras		0.15910.34explicit E-S complexMAPKK-ser
MAPKK*
  Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
5 Enzyme Activity:
Raf-GTP-Ras.1

Enzyme Molecule:
Raf-GTP-Ras		0.15910.34explicit E-S complexMAPKK
MAPKK-ser
  Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
6 Enzyme Activity:
Raf-GTP-Ras.2

Enzyme Molecule:
Raf-GTP-Ras		0.15910.34explicit E-S complexMAPKK-ser
MAPKK*
  Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
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