| | Name | Initial Conc. (uM) | Volume (fL) | Buffered |
| 1 | cGMP | 0 | 0.0016667 | No |
| | |
| 2 | GTP | 1000 | 0.0016667 | No |
| | Under in vivo conditions, GTP is present in excess, ~ 1 mM. |
| 3 | NO | 0 | 0.0016667 | No |
| | Endogenously produced NO concentrations in the course of signal transduction processes are < 100 nM. (Varner et al., Nitric oxide in the nervous system, Academic press, London, UK, pp.191-206.) |
| 4 | NO_a | 0.1 | 0.0016667 | No |
| | |
| 5 | NO_sGCfull_act | 0 | 0.0016667 | No |
| | This is the fully active form of sGC, catalysing the conversion of GTP to cGMP....as proposed by Condorelli et al., 2001, Biophys J, 80:14753-14758. |
| 6 | NO_sGCpart_act | 0 | 0.0016667 | No |
| | This is the partiallly active form of sGC bound with NO, as proposed by Condorelli et al., 2001, Biophys J, 80:2110-2119.. based originally on Zhao et al., 1999, PNAS,96:14753-14758. |
| 7 | sGC_basal | 3 | 0.0016667 | No |
| | Intracellular levels of sGC is around 3 uM, as sGC is enriched in Purkinje cells. (Shinya Kuroda, personal correpondence)... |
