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Molecule Parameter List for R2C2

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*R2C2* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	0	0	1	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
PKA_2003	47	Network	Shared_Object_PKA_2003, PKA, AC, Gs
This model consists of receptor-ligand interaction, G-protein activation, Adenylyl cyclase mediated formation of cAMP and activation of PKA in the neuron. Demonstration programs using this model described in Bhalla US. (2004) Biophys J. 87(2):733-44 to generate a dose-response curve using stochastic calculations are available here.

R2C2 acting as a Molecule in PKA_2003 Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
R2C2	PKA_2003 Accession No. : 47	PKA Pathway No. : 196	0.5	1000	No
This is the R2C2 complex, consisting of 2 catalytic (C) subunits, and the R-dimer. See Taylor et al (1990) Ann Rev Biochem 59:971-1005 for a review. The Doskeland and Ogreid review is better for numbers. Amount of PKA is about .5 uM.

R2C2 acting as a Substrate in a reaction in PKA_2003 Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
cAMP-bind-site-B 1	PKA_2003 Accession No. : 47	PKA Pathway No. : 196	54 (uM^-1 s^-1)	33 (s^-1)	Kd(bf) = 0.6111(uM)	-	Substrate R2C2 cAMP Product cAMP.R2C2
Hasler et al (1992 jun) FASEB J. 6(9):2735-41 say Kd =1e-7M for type II, 5.6e-8 M for type I. Take mean which comes to 2e-13 #/cell Smith et al (1981 Mar) Proc Natl Acad Sci U S A 78(3) 1591-1595 have better data. First kf/kb=2.1e7/M = 3.5e-5 (#/cell).Ogreid and Doskeland (1981 Jul 6) FEBS Lett 129(2) 287-292 have figs suggesting time course of complete assoc is < 1 min.

Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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