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Molecule Parameter List for PLA2*-Ca

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
PLA2*-Ca participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1010001

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • MAPK_MKP1_
    oscillation
  • 9Network
    Shared_Object_MAPK_MKP1_oscillation PKC MAPK 
    PLA2 Ras 
    This model relates to figure 5 in Bhalla US, Iyengar R. Chaos (2001) 11(1):221-226. It includes the model used for figures 2-4 and also has MKP-1 induction by MAPK activity in the synapse. PP2A is set to 0.16 uM and MKP synthesis is varied from 5x to 40 x basal to get a range of interesting behaviours.

    PLA2*-Ca acting as a Molecule in  
    MAPK_MKP1_oscillation Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    PLA2*-Ca
  • MAPK_MKP1_
    oscillation

    Accession No. : 9
  • PLA2
    Pathway No. : 62
    01000No
    Phosphorylated form of PLA2. Still need to hook it up using kinases. PKA: Wightman et al JBC 257 pp6650 1982 PKC: Many refs, eg Gronich et al JBC 263 pp 16645, 1988 but see Lin etal MAPK: Lin et al, Cell 72 pp 269, 1993. Show 3x with MAPK but not PKC alone Do not know if there is a Ca requirement for active phosphorylated state.

    PLA2*-Ca acting as an Enzyme in  
    MAPK_MKP1_oscillation Network
    Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    PLA2*-Ca /
    kenz
  • MAPK_MKP1_
    oscillation

    Accession No. : 9
  • PLA2
    Pathway No. : 62
    201204explicit E-S complexSubstrate
    APC

    Product
    AA
    This form should be 3 to 6 times as fast as the Ca-only form. I have scaled by 4x which seems to give a 5x rise. 10x raise Oct 22 12 x oct 24, changed k2 = 4 * k3

    PLA2*-Ca acting as a Product in a reaction in  
    MAPK_MKP1_oscillation Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
    NameAccession NamePathway NameKfKbKdtauReagents
    PLA2*-Ca-act
  • MAPK_MKP1_
    oscillation

    Accession No. : 9
  • PLA2
    Pathway No. : 62
    6
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.0167(uM)-Substrate
    Ca
    PLA2*

    Product
    PLA2*-Ca
    To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien....



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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