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Enzyme List for pathway MAPK (Pathway Number 182) in Accession MAPK-bistability-fig1c (Accession Number 35) |
Entries are grouped according to Pathway Number and they are alternately color coded using  and  color.Further ordering can be done according to header.   arrow indicates that ordering is done according to ascending or descending order. |
| Enzyme Molecule / Enzyme Activity | Pathway Name / Pathway No. | Km (uM) | kcat (s^-1) | Ratio (k2/k3) | Enzyme Type | Reagents | ||
| 1 | MAPKK* / MAPKKtyr | MAPK Pathway No. 182 | 0.0462963 | 0.15 | 4 | explicit E-S complex | Substrate: MAPK Product : MAPK-tyr | |
| The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, ratio of 4 to get k2=0.6. k1=0.75/46.6nM=2.7e-5 In terms of Michaelis-Menten rates, Km = 0.046, Vmax = 0.15, ratio = 4. | ||||||||
| 2 | MAPKK* / MAPKKthr | MAPK Pathway No. 182 | 0.0462963 | 0.15 | 4 | explicit E-S complex | Substrate: MAPK-tyr Product : MAPK* | |
| Rate consts same as for MAPKKtyr. | ||||||||
| 3 | RGR / RGR.1 | MAPK Pathway No. 182 | 0.159091 | 0.105 | 4 | explicit E-S complex | Substrate: MAPKK Product : MAPKK-ser | |
| Kinetics are the same as for the craf-1* activity, ie., k1=5.5e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. | ||||||||
| 4 | RGR / RGR.2 | MAPK Pathway No. 182 | 0.159091 | 0.105 | 4 | explicit E-S complex | Substrate: MAPKK-ser Product : MAPKK* | |
| Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 5.5e-6, k2 = .42, k3 = 0.105 | ||||||||
| 5 | Raf*-GTP-Ras / Raf*-GTP-Ras.1 | MAPK Pathway No. 182 | 0.159091 | 0.105 | 4 | explicit E-S complex | Substrate: MAPKK Product : MAPKK-ser | |
| Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. They report Km for MAPKK of 0.8 uM. and a Vmax of ~500 fm/min/ug. These parms cannot reach the observed 4X stimulation of MAPK. So we increase the affinity, ie, raise k1 5x to 5.5e-6 which is equivalent to 5-fold reduction in Km to about 0.16. This is, of course, dependent on the amount of MAPKK present. | ||||||||
| 6 | Raf*-GTP-Ras / Raf*-GTP-Ras.2 | MAPK Pathway No. 182 | 0.159091 | 0.105 | 4 | explicit E-S complex | Substrate: MAPKK-ser Product : MAPKK* | |
| Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. | ||||||||