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Enzyme List for pathway Ras (Pathway Number 184) in Accession MAPK-bistability-fig1c (Accession Number 35) |
Entries are grouped according to Pathway Number and they are alternately color coded using  and  color.Further ordering can be done according to header.   arrow indicates that ordering is done according to ascending or descending order. |
| Enzyme Molecule / Enzyme Activity | Pathway Name / Pathway No. | Km (uM) | kcat (s^-1) | Ratio (k2/k3) | Enzyme Type | Reagents | ||
| 1 | GEF* / GEF*-act-ras | Ras Pathway No. 184 | 0.505051 | 0.02 | 4 | explicit E-S complex | Substrate: GDP-Ras Product : GTP-Ras | |
| Kinetics from Orita et al JBC 268(34):25542-25546. Note that the Vmax is slow, but it does match the slow GTP hydrolysis rates. | ||||||||
| 2 | GAP / GAP-inact-ras | Ras Pathway No. 184 | 1.0104 | 10 | 100 | explicit E-S complex | Substrate: GTP-Ras Product : GDP-Ras | |
| From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). The two sets of values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) This is one of the rare cases where we have direct info on the k3 being rate-limiting. Hence the ratio I use for the k2:k3 rates is 100 rather than the usual 4. | ||||||||