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Result: 1 - 6 of 6 rows are displayed

Enzyme List for pathway MAPK (Pathway Number 377) in Accession Ajay_bhalla_2007_ReacDiff1_1e-12 (Accession Number 81)

Entries are grouped according to Pathway Number and they are alternately color coded using  and   color.
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  Enzyme Molecule /

 Enzyme Activity
Pathway Name / 
Pathway No.
Km
(uM)
kcat
(s^-1)
Ratio
(k2/k3)
Enzyme TypeReagents
1 MAPKK* /
MAPKKtyr
MAPK

Pathway No. 377
0.04629640.34explicit E-S complexSubstrate: 
MAPK
Product : 
MAPK-tyr
  The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
2 MAPKK* /
MAPKKthr
MAPK

Pathway No. 377
0.04629640.34explicit E-S complexSubstrate: 
MAPK-tyr
Product : 
MAPK*
  Rate consts same as for MAPKKtyr.
3 Raf-GTP-Ras /
Raf-GTP-Ras.1
MAPK

Pathway No. 377
0.1590960.34explicit E-S complexSubstrate: 
MAPKK
Product : 
MAPKK-ser
  Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
4 Raf-GTP-Ras /
Raf-GTP-Ras.2
MAPK

Pathway No. 377
0.1590960.34explicit E-S complexSubstrate: 
MAPKK-ser
Product : 
MAPKK*
  Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
5 Raf*-GTP-Ras /
Raf*-GTP-Ras.1
MAPK

Pathway No. 377
0.1590960.34explicit E-S complexSubstrate: 
MAPKK
Product : 
MAPKK-ser
  Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
6 Raf*-GTP-Ras /
Raf*-GTP-Ras.2
MAPK

Pathway No. 377
0.1590960.34explicit E-S complexSubstrate: 
MAPKK-ser
Product : 
MAPKK*
  Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6

 
Result: 1 - 6 of 6 rows are displayed



Database compilation and code copyright (C) 2005, Upinder S. Bhalla and NCBS/TIFR
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