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Result: 1 - 9 of 9 rows are displayed

Enzyme List for pathway MAPK (Pathway Number 61) in Accession MAPK_MKP1_oscillation (Accession Number 9)

Entries are grouped according to Pathway Number and they are alternately color coded using  and   color.
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  Enzyme Molecule /

 Enzyme Activity
Pathway Name / 
Pathway No.
Km
(uM)
kcat
(s^-1)
Ratio
(k2/k3)
Enzyme TypeReagents
1 MAPKK* /
MAPKKtyr
MAPK

Pathway No. 61
0.04629630.154explicit E-S complexSubstrate: 
MAPK
Product : 
MAPK-tyr
  The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
2 MAPKK* /
MAPKKthr
MAPK

Pathway No. 61
0.04629630.154explicit E-S complexSubstrate: 
MAPK-tyr
Product : 
MAPK*
  Rate consts same as for MAPKKtyr.
3 Raf-GTP-Ras* /
Raf-GTP-Ras*.1
MAPK

Pathway No. 61
0.1590910.1054explicit E-S complexSubstrate: 
MAPKK
Product : 
MAPKK-ser
  Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
4 Raf-GTP-Ras* /
Raf-GTP-Ras*.2
MAPK

Pathway No. 61
0.1590910.1054explicit E-S complexSubstrate: 
MAPKK-ser
Product : 
MAPKK*
  Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
5 RGR /
RGR.1
MAPK

Pathway No. 61
0.1590910.1054explicit E-S complexSubstrate: 
MAPKK
Product : 
MAPKK-ser
  Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
6 RGR /
RGR.2
MAPK

Pathway No. 61
0.1590910.1054explicit E-S complexSubstrate: 
MAPKK-ser
Product : 
MAPKK*
  Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
7 MKP-1** /
MKP1*-tyr-deph
MAPK

Pathway No. 61
0.066666714Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S
Substrate: 
MAPK-tyr
Product : 
MAPK
  3 Feb 2000. Same rates as MKP-1.
8 MKP-1** /
MKP1*-thr-deph
MAPK

Pathway No. 61
0.066666714Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S
Substrate: 
MAPK*
Product : 
MAPK-tyr
  3 Feb 2000. Same rates as MKP1
9 nuc_MAPK* /
MKP-1-synthesis
MAPK

Pathway No. 61
27.36320.000110explicit E-S complexSubstrate: 
MKP-1-gene
Product : 
MKP-1
  5 Feb 2000 This is a crude model of activation of MKP1 production by MAPK. Time-course poorly constrained: 10 min to > 100 min. Rates are further constrained by the known degradation rates of MKP-1, also in this model. Peak MKP-1 is about 10 times basal (from Brondello et al Science 286:2514 1999). Max MAPK ~.25 uM, degradation 45 min. So this enz has to provide 22nM MKP every 45 min.

 
Result: 1 - 9 of 9 rows are displayed



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