NCBS Home page
Accession List
Pathway List
Search
Authorized Users
Help
News archives

Enter a Search String

Special character and space not allowed in the query term. Search string should be at least 2 characters long.
Search in: Search for Match By


 
Result: 1 - 13 of 13 rows are displayed

Molecule List for pathway AC (Pathway Number 85) in Accession Synaptic_Network (Accession Number 16)

Default ordering is done according to Pathway Number. Table headers can be used for changing the default ordering.
arrow indicates that ordering is done according to ascending or descending order.
The entries are grouped according to Pathway Number and are alternately color coded using  and  color.
  NameAccession
Type
Initial
Conc.

(uM)
Volume
(fL)
BufferedSum Total Of
1 AC1Network0.020No
    AC concentrations are tricky due to poor antibodies. I refer to an estimate from Jacobowitz, PhD Thesis, Mount Sinai School of Medicine around Pg 149 which estimates cyclase as 1/12600 of membrane protein. This gives a whole-cell conc of about 33 nM using assumptions of 2% of cell mass being membrane protein. Defer et al 2000 Am J Physiol Renal Physiol 279:F400-F416 in a good review put AC1 and AC8 (which has similar properties) as among the highly expressed form of brain cyclase. We therefore estimate its levels as a good fraction of the 33 nM, at 20 nM.
2 AC1-CaMNetwork00No
    This state of AC1 is bound to Calmodulin and therefore activated. Gs stimulates it but betagamma inhibits.
3 AC1-GsNetwork00No
    This is the generic Gs-Stimulated state of AC1. Note that the enzyme is normally saturated, so all reactions involving AC1-Gs actually relate to the enzyme-substrate complex.
4 AC2Network0.0150No
    AC concentrations are tricky due to poor antibodies. I refer to an estimate from Jacobowitz, PhD Thesis, Mount Sinai School of Medicine around Pg 149 which estimates cyclase as 1/12600 of membrane protein. This gives a whole-cell conc of about 33 nM using assumptions of 2% of cell mass being membrane protein. Defer et al 2000 Am J Physiol Renal Physiol 279:F400-F416 in a good review put AC2 among the highly expressed form of brain cyclase. We therefore estimate its levels as a good fraction of the 33 nM, at 15 nM. This actually adds up to a little more than 33, but it is well within error estimates.
5 AC2*Network00No
    This is the phosphorylation-activated form of AC2.
6 AC2*-GsNetwork00No
    This is the form activated synergistically by phosphorylation as well as Gs binding.
7 AC2-GsNetwork00No
    This is the generic Gs-Stimulated form of AC2
8 AMPNetwork100Yes
    AMP is a tightly regulated metabolite, so here we simply buffer it to its resting value. The value doesn't really matter to any of the calculations since it acts like a one-way sink.
9 ATPNetwork50000Yes
    ATP is present in all cells between 2 and 10 mM. See Lehninger.
10 CaM.PDE1Network00No
    PDE1 is the 66 KDa form which binds CaM. Borisy et al J Neurosci 12(3):915-923 report a 6x stimulation with calcium.
11 cAMP-PDENetwork0.450No
    The levels of the PDE are not known at this time. However, enough kinetic info and info about steady-state levels of cAMP etc are around to make it possible to estimate this.
12 cAMP-PDE*Network00No
    This form has about 2X activity as plain PDE. See Sette et al JBC 269:28 18271-18274 1994.
13 PDE1Network20No
    CaM-Dependent PDE. 66KDa Borisy et al J Neurosci 12(3):915-923 About 0.6% of membrane protein. so estimate 2 uM as conc in brain. Also see Conti et al Adv Sec Mess Phosphoprotein Res 1992 25:87-99

 
Result: 1 - 13 of 13 rows are displayed



Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
This Copyright is applied to ensure that the contents of this database remain freely available. Please see FAQ for details.