| | Molecule Name/
Site Name | Km (uM) | kcat (1/s) | Ratio
(k2/k3) | Enzyme Type | Substrate | Product |
| 1 |
Enzyme Activity:
Cdh1_i_k2_prime
Enzyme Molecule:
Cdh1_i | 100.002 | 5 | 4 | explicit E-S complex | CycB
| degraded
|
| | k2_prime = 0.05. so actually this reaction is pretty negligible. rate = k2_prime * Cdh1_i * CycB From MM kinetics, rate = kcat * Cdh1_i * CycB / (CycB + Km). Let Km >>CycB, so Km = 10. Then kcat = k2_prime * Km = 0.5 |
| 2 |
Enzyme Activity:
Cdh1_k2
Enzyme Molecule:
Cdh1 | 100 | 2000 | 4 | explicit E-S complex | CycB
| degraded
|
| | k2 = 20 Let Km = 100, so it is >> substrate. Then kcat = Km * k2 = 2000 |
| 3 |
Enzyme Activity:
k3_prime
Enzyme Molecule:
k3_prime | 0.01 | 7.5 | 4 | explicit E-S complex | Cdh1_i
| Cdh1
|
| | k3_prime = 7.5 J3 = Km = 0.01 Rate = [k3_prime] * [Cdh1_i]/(J3 + [Cdh1_i]) |
