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Reaction List for pathway NOS (Pathway Number 65) | | Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reactions is not considered. |
| | Name | Kf | Kb | Kd | tau | Substrate | Product | | 1 |
Ca-CaMbind-nNOS | kf
(uM^-1 s^-1) | 0.05
(s^-1) | Kd(bf) = 0.0154(uM) | - | nNOS
CaM-Ca4
| Ca-CaM_nNOS
| | | Those binding CaM have a high Kd, including nNOS, ~ <=10 nM. Calcium mediated nNOS activity is required for NO synthesis. Rates from experiments... The binding of CaM to NOS has been demonstrated to act as the trigger necessary for electron transfer and catalytic activity.(Marletta, Biochemistry, 1997;36:12337-12345) | | 2 |
k1 | kf
(s^-1) | 0
(s^-1) | - | - | nNOS.arg
| nNOS.OH.Arg
| | | Rates from Iwanaga et al., Biochem, 1999;38(50):16629-16635. Obtained from rapid quenching expts at 25 C. >>>>>> 25 +/- 2.0 | | 3 |
k2 | kf
(s^-1) | 0
(s^-1) | - | - | nNOS.OH.Arg
| OH.Arg
| | | Rates from Biochem., 1999, 38(50), 16629-16635. obtained from rapid quenching expts at 25 C.... >>>>>> 4.4 +/- 0.5 | | 4 |
k3 | kf
(s^-1) | 0
(s^-1) | - | - | nNOS.OH.Arg
| nNOS.Cit+NO
| | | Rate from Biochem., 1999, 38(50), 16629-16635. Obtained from rapid queching expts at 25 C >>>>>> 19 +/- 3.0 | | 5 |
k4 | kf
(s^-1) | 0
(uM^-1 s^-1) | - | - | nNOS.Cit+NO
| Cit
NO
| | | Rate from Biochem., 1999, 38(50), 16629-16635., from rapid quenching expts at 25 C >>>>>> 2.7 +/- 0.3 | | 6 |
nNOSbindArg | kf
(uM^-1 s^-1) | 81
(s^-1) | Kd(bf) = 162(uM) | - | L-Arg
Ca-CaM_nNOS
| nNOS.arg
| | | From Stuehr et al., Biochem,1999,38(38):12446-12451. Steuhr et al., from stopped flow expts of sub binding to nNOS report 0.5 (k-1) and 81 (k2). Rates used straight from results. |
Pathway Details Molecule List Enzyme List Reaction List
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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