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Molecule Parameter List for Ca-CaM_nNOS | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | | NOS_Iwanaga | 11 | Pathway |
NOS | | This model is based on Iwanaga T et al. Biochemistry (1999) 38(50):16629-35.The model features the catalytic activity of neuronal Nitric Oxide Synthase expressed in Escherichia coli in a four step reaction mechanism. The rates found in the model are used directly from their paper and have been determined from experiments performed at 25 C. |
Ca-CaM_nNOS acting as a Molecule in NOS_Iwanaga Network
| Name | Accession Name | Pathway Name | Initial Conc.
(uM) | Volume
(fL) | Buffered | | Ca-CaM_nNOS | NOS_Iwanaga
Accession No. : 11 | NOS
Pathway No. : 65 | 0 | 0.0016667 | No | | The CaM-Ca bound nNOS, which is the acitve form. |
Ca-CaM_nNOS acting as a Substrate in a reaction in NOS_Iwanaga Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | nNOSbindArg | NOS_Iwanaga
Accession No. : 11 | NOS
Pathway No. : 65 | 0.5
(uM^-1 s^-1) | 81
(s^-1) | Kd(bf) = 162(uM) | - | Substrate
Ca-CaM_nNOS
L-Arg
Product
nNOS.arg
| | From Stuehr et al., Biochem,1999,38(38):12446-12451. Steuhr et al., from stopped flow expts of sub binding to nNOS report 0.5 (k-1) and 81 (k2). Rates used straight from results. |
Ca-CaM_nNOS acting as a Product in a reaction in NOS_Iwanaga Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | Ca-CaMbind-nNOS | NOS_Iwanaga
Accession No. : 11 | NOS
Pathway No. : 65 | 3.25
(uM^-1 s^-1) | 0.05
(s^-1) | Kd(bf) = 0.0154(uM) | - | Substrate
CaM-Ca4
nNOS
Product
Ca-CaM_nNOS
| | Those binding CaM have a high Kd, including nNOS, ~ <=10 nM. Calcium mediated nNOS activity is required for NO synthesis. Rates from experiments... The binding of CaM to NOS has been demonstrated to act as the trigger necessary for electron transfer and catalytic activity.(Marletta, Biochemistry, 1997;36:12337-12345) |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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