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Molecule Parameter List for mGluR

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

mGluR participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	0	0	2	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
NonOsc_Ca_ IP3metabolism	23	Network	MIPP,  CaMKII,  CaM,   PKC,  IP3-3K,  CaRegulation,   Gq,  PLCbeta,  134_dephos,   145_dephos,  IP4-system,  IHP-system,   1345_dephos
This network models detailed metabolism of Ins(145)P3, integrated with GPCR mediated PLCbeta activation and Ca release by the InsP3 receptor in the neuron. The calcium response is non-oscillatory. Mishra J, Bhalla US. Biophys J. 2002 Sep;83(3):1298-316.

mGluR acting as a Molecule in  NonOsc_Ca_IP3metabolism Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
mGluR	NonOsc_Ca_ IP3metabolism Accession No. : 23	Gq Pathway No. : 111	0.3	1000	No
From Mahama and Linderman, Total # of receptors/cell = 1900 However, the density is likely to be very high at the synapse. Fay et al Biochem 30 5066-5075 1991 have a value of 60K receptors per cell for neutrophils which comes to 0.1 uM. Here we have a situation where trying to represent the synapse by a 10 micron cube gives awkward results. I will scale up to 0.3 uM since synaptic receptor density is likely to be higher, with the caveat that I should really be using a more geometrically realistic model.

mGluR acting as a Substrate in a reaction in  NonOsc_Ca_IP3metabolism Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	RecLigandBinding	NonOsc_Ca_ IP3metabolism Accession No. : 23	Gq Pathway No. : 111	16.8 (uM^-1 s^-1)	10 (s^-1)	Kd(bf) = 0.5952(uM)	-	Substrate Glu mGluR Product Rec-Glu
	From Martin et al FEBS Lett 316:2 191-196 1993 we have Kd = 600 nM Assuming kb = 10/sec, we get kf = 10/(0.6 uM * 6e5) = 2.8e-5 1/sec/# The off time for Glu seems pretty slow: Nicoletti et al 1986 PNAS 83:1931-1935 and Schoepp and Johnson 1989 J Neurochem 53 1865-1870 indicate it is at least 30 sec. Here we are a little faster because this is only a small part of the off rate, the rest coming from the Rec-Gq complex.
2	Rec-bind-Gq	NonOsc_Ca_ IP3metabolism Accession No. : 23	Gq Pathway No. : 111	0.6 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 1.6667(uM)	-	Substrate G-GDP mGluR Product Rec-Gq
	From Berstein et al 1992 JBC 267(12):8081-8088 we know that 15-40% of Gq binds, GTP_gamma_S. Also about 20-30% of Gq is bound to GTP. To get to these values the receptor-Gq amount should be similar. These rates are designed to give that steady state with a fast tau of 1 sec.