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Molecule Parameter List for MKKK-P

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

MKKK-P participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	2	1	1	0	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
MAPK_osc	27	Pathway	MAPK
This MAPK model is based on Boris N. Kholodenko Eur J Biochem. (2000) 267(6):1583-8 for data from Xenopus oocytes extracts.

MKKK-P acting as a Molecule in  MAPK_osc Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
MKKK-P	MAPK_osc Accession No. : 27	MAPK Pathway No. : 139	0	0.0016667	No
This is the phosphorylated form of MKKK which converts MKK to MKK-P and then to MKK-PP from Kholodenko, 2000.

MKKK-P acting as an Enzyme in  MAPK_osc Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	MKKK-P / 3	MAPK_osc Accession No. : 27	MAPK Pathway No. : 139	0.0150001	0.025	4	Classical Michaelis-Menten V = Etot.S.Kcat/Km+S	Substrate MKK Product MKK-P
	Km is 15 nM and Vmax is 0.025s-1 from Kholodenko, 2000
2	MKKK-P / 4	MAPK_osc Accession No. : 27	MAPK Pathway No. : 139	0.0150001	0.025	4	Classical Michaelis-Menten V = Etot.S.Kcat/Km+S	Substrate MKK-P Product MKK-PP
	Km is 15nM and Vmax is 0.025s-1 from Kholodenko, 2000.

MKKK-P acting as a Substrate for an Enzyme in  MAPK_osc Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
int1  / 2	MAPK_osc Accession No. : 27	MAPK Pathway No. : 139	0.008	0.25	4	Classical Michaelis-Menten V = Etot.S.Kcat/Km+S	Substrate MKKK-P Product MKKK
Km is 8nM and Vmax is 0.25nM.s-1 from Kholodenko, 2000.

MKKK-P acting as a Product of an Enzyme in  MAPK_osc Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
Ras-MKKKK  / 1	MAPK_osc Accession No. : 27	MAPK Pathway No. : 139	0.01	2.5	4	Classical Michaelis-Menten V = Etot.S.Kcat/Km+S	Substrate MKKK Product MKKK-P
The Km is 10nM and Vmax is 2.5nM sec^-1. We assume that there is 1 nM of the Ras-MKKKK. From Kholodenko, 2000. If the enzymes are not flagged as Available, then this Km should be set to 0.1 to obtain oscillations.