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Molecule Parameter List for sGC | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
| sGC participated as | Molecule | Sum total of | Enzyme | Substrate of an enzyme | Product of an enzyme | Substrate in Reaction | Product in Reaction | | No. of occurrences | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | CO_activation_
of_GC | 28 | Pathway |
sGC | Carbon Monoxide is an activator of soluble Guanylyl Cyclase and has been implicated as a neuronal messenger [Ingi T. et al. Neuron (1996) 16(4):835-42].
CO binds to the heme group on sGC, similar to NO binding. Exogenous CO at similar conc. to endogenous levels were used to study the extent of activation of GC. Olfactory receptor neurons were used by Ingi et al., to investigate the relationship of CO to cGMP levels, as these cells have high levels of HO activity but no NOS activity.
Kharitonov VG. et al. Proc Natl Acad Sci U S A. (1995) 92(7):2568-71 and
Kharitonov VG. et al. Biochemistry (1999) 38(33):10699-706 report the presence of a six coordinate and a five coordinate intermediate of carboxy GC, induced by CO. Considering that activation of sGC by CO is similar in almost all tissues, some rates have been taken from original published works cited as references in Kharitonov et al., and Ingi et al., the primary datasources, this model is based on. |
sGC acting as a Molecule in CO_activation_of_GC Network
| Name | Accession Name | Pathway Name | Initial Conc.
(uM) | Volume
(fL) | Buffered | | sGC | CO_activation_
of_GC
Accession No. : 28 | sGC
Pathway No. : 140 | 3 | 0.0016667 | No | | sGC is enriched in purkine cells ~3 uM (Conc from Shinya Kuroda, assumed on the basis of reported data in Ariano et al., 1982, PNAS,79: 297-300) soluble Guanylyl Cyclase localization is virtually identical to that of HO-2 in numerous brain regions.(Ingi et al., 1995, J Neurosci., 15:8214-8222), in contrast to discrepancies in the localization of NOS and sGC in the brain. |
sGC acting as a Substrate in a reaction in CO_activation_of_GC Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | CObindsGCa | CO_activation_
of_GC
Accession No. : 28 | sGC
Pathway No. : 140 | 0.12
(uM^-1 s^-1) | 28
(s^-1) | Kd(bf) = 233.3333(uM) | - | Substrate
CO
sGC
Product
GC6-CO
| | This gives rise to the six co-ordinate carboxy GC. Kharitonov et al., 1995, PNAS, 92:2568-2571 report CO association and dissociation rates at 1.2e5 /M/sec and 28 /sec respectively. Kharitonov et al., 1999, Biochemistry, 38(33):10699-10706 report Ka at 7.86 +- 0.50 /mM. |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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