NCBS Home page
Accession List
Pathway List
Search
Authorized Users
Help
News archives

Enter a Search String

Special character and space not allowed in the query term. Search string should be at least 2 characters long.
Search in: Search for Match By

Molecule Parameter List for R2C2

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
R2C2 participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1000010

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
fig4_synapse3Network
Shared_Object_fig4_synapse PKC PLA2 
PLCbeta Gq MAPK 
Ras CaMKII CaM 
PP1 PP2B PKA 
AC 
This is the composite model of 4 kinases: PKC, MAPK, PKA and CaMKII and numerous regulatory pathways involved in synaptic signaling. From Bhalla US and Iyengar R. Science (1999) 283(5400):381-7.This model comes from figure 4 of that paper.
Demonstration script files for generating the figures in the paper, including figure 4, are available here.

R2C2 acting as a Molecule in  
fig4_synapse Network
NameAccession NamePathway NameInitial Conc.
(uM)
Volume
(fL)
Buffered
R2C2fig4_synapse
Accession No. : 3
PKA
Pathway No. : 30
0.51000No
PKA: Protein kinase activated by cAMP.
R2C2 complex consists of 2 catalytic (C) subunits, and the R-dimer. There are two types of cAMP binding sites termed A and B according to the rate with which they exchange bound labelled nucleotide.
Dagfinn Ogreid and Stein Ove Doskeland (1981) Int.J.Biochem. 13(1):1-19
Physiological concentration of PKA = 0.59 uM (cardiac myocyte)
Jeffrey J. Saucerman et al (2003) J.Biol.Chem. 278(48):47997-48003

R2C2 acting as a Substrate in a reaction in  
fig4_synapse Network
Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
NameAccession NamePathway NameKfKbKdtauReagents
  • cAMP-bind-site-B
    1
  • fig4_synapse
    Accession No. : 3
    PKA
    Pathway No. : 30
    54
    (uM^-1 s^-1)
    33
    (s^-1)
    Kd(bf) = 0.6111(uM)-Substrate
    R2C2
    cAMP

    Product
    R2C2-cAMP
    Kf = 54 /sec/uM, Kb = 33 /sec; PKA in normal human T lymphocytes. Hasler et al (1992) FASEB J 6:2735-2741
    Kd =1e-07 M for type II, 5.6e-08 M for type I; Stephen B. Smith et al (1981) PNAS, USA 78: 1591-1595
    Ka1 = 2.1e+07 /M which gives Kd = 47 nM, Kan = 5e+08 /M or Kd of 2nM from Fig.7



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
    This Copyright is applied to ensure that the contents of this database remain freely available. Please see FAQ for details.