Enter a Search String

Special character and space not allowed in the query term. Search string should be at least 2 characters long.

Search in:

ALL Accession Pathway Molecule Enzyme Reaction Connected to

Search for

Match

Anything Exact Word Starting With

By

Name Notes Model

Molecule Parameter List for E2

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

E2 participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	0	0	1	1

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
SERCA	37	Pathway	SERCA
All the constants are from: Mahaney JE. et al. Biophys J. (2000) 78(3) 1306-23. New Insights on cardiac Ca-ATPase (expressed in Sf21 cells) regulation by Phosphlamban.

E2 acting as a Molecule in  SERCA Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
E2	SERCA Accession No. : 37	SERCA Pathway No. : 187	0.1509	0.0016667	No
Concentration was calculatd from the literature source Mahaney et al. ( 2000 Mar) Biophys J. 78(3) 1306-23. In case of EP formation and Pi liberation E2 is set to calculated concentration (0.1509uM), in case EP decompostion E2 is set to 0uM. Calculations of initial SERCA2a concentration: Data given: 8.3% of the total protein is SERCA2a. 0.2mg of SF21 microsome was taken. Molecular wt of SERCA2a is 110,000 Da. Thus E2 conc in moles is (8.3 * 0.2 * 1e-6 ) / 11 = 0.1509uM.

E2 acting as a Substrate in a reaction in  SERCA Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
reac1	SERCA Accession No. : 37	SERCA Pathway No. : 187	0.35 (s^-1)	0.35 (s^-1)	Keq = 1(uM)	1.429sec	Substrate E2 Product E1
kf and kb are the picked up from the Table-6 of literature source Mahaney et al. (2000 Mar) Biophys J. 78(3) 1306-23. .

E2 acting as a Product in a reaction in  SERCA Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
reac8	SERCA Accession No. : 37	SERCA Pathway No. : 187	0.4 (s^-1)	0 (uM^-1 s^-1)	-	-	Substrate EPi Product E2 pi
kf and kb were taken from the literature source Mahaney et al. (2000 Mar) Biophys J. 78(3) 1306-23. some minor adjustments for step7 and step8 were required to produce a precise simulation of the EP formation and Pi liberation time course as claimed by the author of above literature.