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Molecule Parameter List for E2 | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
E2 acting as a Molecule in SERCA Network
| Name | Accession Name | Pathway Name | Initial Conc.
(uM) | Volume
(fL) | Buffered | | E2 | SERCA
Accession No. : 37 | SERCA
Pathway No. : 187 | 0.1509 | 0.0016667 | No | Concentration was calculatd from the literature source
Mahaney et al. ( 2000 Mar) Biophys J. 78(3) 1306-23.
In case of EP formation and Pi liberation E2 is set to calculated concentration (0.1509uM), in case EP decompostion E2 is set to 0uM.
Calculations of initial SERCA2a concentration:
Data given: 8.3% of the total protein is SERCA2a. 0.2mg of SF21 microsome was taken. Molecular wt of SERCA2a is 110,000 Da. Thus E2 conc in moles is (8.3 * 0.2 * 1e-6 ) / 11 = 0.1509uM. |
E2 acting as a Substrate in a reaction in SERCA Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
E2 acting as a Product in a reaction in SERCA Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | reac8 | SERCA
Accession No. : 37 | SERCA
Pathway No. : 187 | 0.4
(s^-1) | 0
(uM^-1 s^-1) | - | - | Substrate
EPi
Product
E2
pi
| kf and kb were taken from the literature source
Mahaney et al. (2000 Mar) Biophys J. 78(3) 1306-23.
some minor adjustments for step7 and step8 were required to produce a precise simulation of the EP formation and Pi liberation time course as claimed by the author of above literature. |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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