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Molecule Parameter List for EP

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

EP participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	0	0	1	1

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
SERCA	37	Pathway	SERCA
All the constants are from: Mahaney JE. et al. Biophys J. (2000) 78(3) 1306-23. New Insights on cardiac Ca-ATPase (expressed in Sf21 cells) regulation by Phosphlamban.

EP acting as a Molecule in SERCA Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
EP	SERCA Accession No. : 37	SERCA Pathway No. : 187	0	0.0016667	No
Formation and decomposition of phosphoenzyme is monitured on a time course under 3 condition. 1. SERCA2a alone. 2. SERCA2a + Wild type phospholamban. 3. SERCA2a + L37A phospholamban.

EP acting as a Substrate in a reaction in SERCA Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
reac7	SERCA Accession No. : 37	SERCA Pathway No. : 187	0.3 (s^-1)	0.7 (s^-1)	Keq = 2.3333(uM)	1sec	Substrate EP Product EPi
kf and kb were taken from the literature source Mahaney et al. (2000 Mar) Biophys J. 78(3) 1306-23. Some minor adjustments for steps 7 and 8 were required to prduce a precise simulation of the EP formation and Pi liberation time course

EP acting as a Product in a reaction in SERCA Network

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
reac6	SERCA Accession No. : 37	SERCA Pathway No. : 187	35 (s^-1)	0 (uM^-1 s^-1)	-	-	Substrate CaE1_prime_ATP Product ADP EP
kf and kb were directly taken from the Table-6 of literature source Mahaney et al. (2000 Mar) Biophys J. 78(3) 1306-23.

Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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