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Molecule Parameter List for Ca | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
| Ca participated as | Molecule | Sum total of | Enzyme | Substrate of an enzyme | Product of an enzyme | Substrate in Reaction | Product in Reaction | | No. of occurrences | 1 | 0 | 0 | 0 | 0 | 2 | 0 |
Accession and Pathway Details | |
Ca acting as a Molecule in SERCA Network
| Name | Accession Name | Pathway Name | Initial Conc.
(uM) | Volume
(fL) | Buffered | | Ca | SERCA
Accession No. : 37 | SERCA
Pathway No. : 187 | 0.268 | 0.0016667 | Yes | | For simulation of EP formation and Pi liberation time couse at various [ca2+] the concentrations were obtained from the literature source Mahaney et al. Biophysical Journal 78(3) 1306-1323 ******************************************************************** In case of EP decomposition after phosphorylation with 1uM ATP for 30s at 0 deg centigrade. At 30s 5mM EGTA (final conc) was added to the reaction mixture to remove all traces of ionized Ca2+ form the reaction mixture and thus to reduced the ionized [Ca2+]. Actual concentration of left over calcium was calculated using EGTA - Free Calcium concentration calculator - a web based program by entering the experimental constants furnished in the literature source Mahaney et al Biophysical Journal 78(3) 1306-1323. Calculator program can be found at:- http://www.stanford.edu/~cpatton/webmaxclitel15.htm ************************************************************ |
Ca acting as a Substrate in a reaction in SERCA Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| | Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | 1 | reac2 | SERCA
Accession No. : 37 | SERCA
Pathway No. : 187 | 50
(uM^-1 s^-1) | 120
(s^-1) | Kd(bf) = 2.4(uM) | - | Substrate
Ca
E1
Product
CaE1
| | | Kinetic constants for this step had substantial effects on the steady state EP level.
For SERCA2a alone the kf was 200uM
SERCA2a + Wild type Phospholamban kf was reduced to 100uM.
SERCA2a + L37A Phospholamban kf was reduced to 50um as specified by the author ( Mahaney et al. (2000 Mar) Biophys J. 78(3) 1306-23.).
kb in all the cases were maintained at 120/s. | | 2 | reac4 | SERCA
Accession No. : 37 | SERCA
Pathway No. : 187 | 80
(uM^-1 s^-1) | 3.2
(s^-1) | Kd(bf) = 0.04(uM) | - | Substrate
Ca
CaE1_prime
Product
Ca2E1_prime
| | | kf and kb were taken from the Table-6 of literature source
Mahaney et al. (2000 Mar) Biophys J. 78(3) 1306-23.
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| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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