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Molecule Parameter List for Ca | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by  color. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Statistics | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Ca participated as | Molecule | Sum total of | Enzyme | Substrate of an enzyme | Product of an enzyme | Substrate in Reaction | Product in Reaction |
| No. of occurrences | 1 | 0 | 0 | 0 | 0 | 2 | 0 |
Accession and Pathway Details |
| Accession Name | Accession No. | Accession Type | Pathway Link |
| SERCA | 37 | Pathway | SERCA |
| All the constants are from: Mahaney JE. et al. Biophys J. (2000) 78(3) 1306-23. New Insights on cardiac Ca-ATPase (expressed in Sf21 cells) regulation by Phosphlamban. | |||
Ca acting as a Molecule in SERCA Network
| Name | Accession Name | Pathway Name | Initial Conc. (uM) | Volume (fL) | Buffered | |
| Ca | SERCA Accession No. : 37 | SERCA Pathway No. : 187 | 0.268 | 0.0016667 | Yes | |
| For simulation of EP formation and Pi liberation time couse at various [ca2+] the concentrations were obtained from the literature source Mahaney et al. Biophysical Journal 78(3) 1306-1323 ******************************************************************** In case of EP decomposition after phosphorylation with 1uM ATP for 30s at 0 deg centigrade. At 30s 5mM EGTA (final conc) was added to the reaction mixture to remove all traces of ionized Ca2+ form the reaction mixture and thus to reduced the ionized [Ca2+]. Actual concentration of left over calcium was calculated using EGTA - Free Calcium concentration calculator - a web based program by entering the experimental constants furnished in the literature source Mahaney et al Biophysical Journal 78(3) 1306-1323. Calculator program can be found at:- http://www.stanford.edu/~cpatton/webmaxclitel15.htm ************************************************************ | ||||||
Ca acting as a Substrate in a reaction in SERCA Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | |
| 1 | reac2 | SERCA Accession No. : 37 | SERCA Pathway No. : 187 | 50 (uM^-1 s^-1) | 120 (s^-1) | Kd(bf) = 2.4(uM) | - | Substrate Ca E1 Product CaE1 |
| Kinetic constants for this step had substantial effects on the steady state EP level. For SERCA2a alone the kf was 200uM SERCA2a + Wild type Phospholamban kf was reduced to 100uM. SERCA2a + L37A Phospholamban kf was reduced to 50um as specified by the author ( Mahaney et al. (2000 Mar) Biophys J. 78(3) 1306-23.). kb in all the cases were maintained at 120/s. | ||||||||
| 2 | reac4 | SERCA Accession No. : 37 | SERCA Pathway No. : 187 | 80 (uM^-1 s^-1) | 3.2 (s^-1) | Kd(bf) = 0.04(uM) | - | Substrate Ca CaE1_prime Product Ca2E1_prime |
| kf and kb were taken from the Table-6 of literature source Mahaney et al. (2000 Mar) Biophys J. 78(3) 1306-23. | ||||||||