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Molecule Parameter List for E0*

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

E0* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	1	0	0	0	2

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
MAPK_Asthagiri_ no_fb	44	Pathway	MAPK_Asthagiri_no_fb
This is a complex model to examine mechanisms that govern MAPK pathway dynamics in Chinese hamster ovary (CHO) cell lines. This model simulates the results as per the figure 4 of the paper by Asthagiri AR and Lauffenburger DA. Biotechnol Prog. (2001) 17(2):227-39.

E0* acting as a Molecule in MAPK_Asthagiri_no_fb Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
E0*	MAPK_Asthagiri_ no_fb Accession No. : 44	MAPK_Asthagiri_ no_fb Pathway No. : 192	0	390	No

E0* acting as an Enzyme in MAPK_Asthagiri_no_fb Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
E0* / E1_activ	MAPK_Asthagiri_ no_fb Accession No. : 44	MAPK_Asthagiri_ no_fb Pathway No. : 192	0.0600001	0.1	5	explicit E-S complex	Substrate E1 Product E1*
k1+ =610^8 M-1 min-1, k1- =30 min-1, kcat,1 =6 min-1 and km caluculated to 0.06 from Anand R. Asthagiri and Douglas A. Lauffenburger (2001 Mar-Apr) Biotechnol Prog. 17(2):227-39*

E0* acting as a Product in a reaction in MAPK_Asthagiri_no_fb Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	C*A1-bind-A2	MAPK_Asthagiri_ no_fb Accession No. : 44	MAPK_Asthagiri_ no_fb Pathway No. : 192	5.0001 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.1(uM)	-	Substrate A2 CA1 Product* E0*
	kf2 and kr2 are 310^8 M-1 min-1 and 0.5/sec from Anand R. Asthagiri and Douglas A. Lauffenburger (2001 Mar-Apr) Biotechnol Prog. 17(2):227-39*
2	C*-bind-A1A2	MAPK_Asthagiri_ no_fb Accession No. : 44	MAPK_Asthagiri_ no_fb Pathway No. : 192	5.0001 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.1(uM)	-	Substrate A1A2 C* Product E0*
	Kf12 and Kr12 are 310^8 M-1 min-1 and 0.5/sec from Anand R. Asthagiri and Douglas A. Lauffenburger (2001 Mar-Apr) Biotechnol Prog. 17(2):227-39*

Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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