Enter a Search String

Special character and space not allowed in the query term. Search string should be at least 2 characters long.

Search in:

ALL Accession Pathway Molecule Enzyme Reaction Connected to

Search for

Match

Anything Exact Word Starting With

By

Name Notes Model

Molecule Parameter List for PLA2-cytosolic

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

PLA2-cytosolic participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	1	0	2	1

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
MAPK_network_ 2003	50	Network	Shared_Object_MAPK_network_2003,  PKC,  PLA2,   PLCbeta,  Gq,  MAPK,   Ras,  EGFR,  Sos,   PLC_g,  CaMKII,  CaM,   PP1,  PP2B,  PKA,   AC
This is a network model of many pathways present at the neuronal synapse. The network has properties of temporal tuning as well as steady-state computational properties. In its default form the network is bistable.Bhalla US Biophys J. 2004 Aug;87(2):745-53

PLA2-cytosolic acting as a Molecule in  MAPK_network_2003 Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
PLA2-cytosolic	MAPK_network_ 2003 Accession No. : 50	PLA2 Pathway No. : 208	0.4	1000	No
Calculated cytosolic was 20 nm from Wijkander and Sundler However, Leslie and Channon use about 400 nM. Need to confirm, but this is the value I use here. Another recalc of W&S gives 1uM

PLA2-cytosolic acting as a Substrate for an Enzyme in  MAPK_network_2003 Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
MAPK*  / MAPK*	MAPK_network_ 2003 Accession No. : 50	Shared_Object_ MAPK_network_ 2003 Pathway No. : 206	25.641	20	4	explicit E-S complex	Substrate PLA2-cytosolic Product PLA2*
Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5

PLA2-cytosolic acting as a Substrate in a reaction in  MAPK_network_2003 Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	PLA2-Ca-act	MAPK_network_ 2003 Accession No. : 50	PLA2 Pathway No. : 208	1 (uM^-1 s^-1)	0.1 (s^-1)	Kd(bf) = 0.1(uM)	-	Substrate Ca PLA2-cytosolic Product PLA2-Ca*
	Leslie and Channon BBA 1045 (1990) 261-270 fig6 pp267.
2	PIP2-PLA2-act	MAPK_network_ 2003 Accession No. : 50	PLA2 Pathway No. : 208	0.0012 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 416.6667(uM)	-	Substrate PLA2-cytosolic temp-PIP2 Product PIP2-PLA2*

PLA2-cytosolic acting as a Product in a reaction in  MAPK_network_2003 Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
dephosphorylate- PLA2*	MAPK_network_ 2003 Accession No. : 50	PLA2 Pathway No. : 208	0.17 (s^-1)	0 (s^-1)	-	-	Substrate PLA2* Product PLA2-cytosolic