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Molecule Parameter List for AC2*

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

AC2* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	1	0	1	2	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
MAPK_network_ 2003	50	Network	Shared_Object_MAPK_network_2003,  PKC,  PLA2,   PLCbeta,  Gq,  MAPK,   Ras,  EGFR,  Sos,   PLC_g,  CaMKII,  CaM,   PP1,  PP2B,  PKA,   AC
This is a network model of many pathways present at the neuronal synapse. The network has properties of temporal tuning as well as steady-state computational properties. In its default form the network is bistable.Bhalla US Biophys J. 2004 Aug;87(2):745-53

AC2* acting as a Molecule in  MAPK_network_2003 Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
AC2*	MAPK_network_ 2003 Accession No. : 50	AC Pathway No. : 221	0	1000	No
This version is activated by Gs and by a betagamma and phosphorylation.

AC2* acting as an Enzyme in  MAPK_network_2003 Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
AC2* / kenz	MAPK_network_ 2003 Accession No. : 50	AC Pathway No. : 221	20.1149	7	4	Classical Michaelis-Menten V = Etot.S.Kcat/Km+S	Substrate ATP Product cAMP
Reduced Km to match expt data for basal activation of AC2 by PKC. Now k1 = 2.9e-6, k2 = 72, k3 = 18

AC2* acting as a Product of an Enzyme in  MAPK_network_2003 Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
PKC-active  / phosph-AC2	MAPK_network_ 2003 Accession No. : 50	Shared_Object_ MAPK_network_ 2003 Pathway No. : 206	33.3333	4	4	explicit E-S complex	Substrate AC2 Product AC2*
Phorbol esters have little effect on AC1 or on the Gs-stimulation of AC2. So in this model we are only dealing with the increase in basal activation of AC2 induced by PKC k1 = 1.66e-6 k2 = 16 k3 =4

AC2* acting as a Substrate in a reaction in  MAPK_network_2003 Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	dephosph-AC2	MAPK_network_ 2003 Accession No. : 50	AC Pathway No. : 221	0.1 (s^-1)	0 (s^-1)	-	-	Substrate AC2* Product AC2
	Random rate.
2	Gs-bind-AC2*	MAPK_network_ 2003 Accession No. : 50	AC Pathway No. : 221	833.28 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 0.0012(uM)	-	Substrate AC2* Gs-alpha Product AC2*-Gs
	kb/kf = 1.2 nM so kf = kb/720 = 1.3888 * kb.