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Molecule Parameter List for RP

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

RP participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	1	0	1	0	3	6

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
EGFR_Signaling_ pathway	52	Pathway	EGFR
This model is based on the model and parameters published in paper: Quantification of Short Term Signaling by the EGFR Kholodenko et al, The J Biol Chem. , Vol 274, No. 42, Issue of October 15, pp. 30169 - 30181, 1999. Note: There are a few small ambiguities in the paper about initial conditions employed for obtaining the results. Thanks to Dr. Kholodenko for clarifying those ambiguities and providing the values used in the simulations:[G-S]=33.04579, [Grb]=51.95431, [SOS]=0.95421.

RP acting as a Molecule in EGFR_Signaling_pathway Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
RP	EGFR_Signaling_ pathway Accession No. : 52	EGFR Pathway No. : 223	0	1.6667E-6	No
Taken from Kholodenko BN et al J Biol Chem. (1999) 274(42):30169-81.

RP acting as a Summed Molecule in EGFR_Signaling_pathway Network

Accession Name	Pathway Name	Target	Input
EGFR_Signaling_ pathway Accession No. : 52	EGFR Pathway No. : 223	pEGFRtot	RP R-PL R-PLP R-G R-G-S R-Sh R-ShP R-Sh-G R-Sh-G-S
Taken from Kholodenko BN et al J Biol Chem. (1999) 274(42):30169-81. Total phosphorylated EGFR = 2.([RP] + [R-PL] + [R-PLP] + [R-G] + [R-G-S] + [R-Sh] + [R-ShP] + [R-Sh-G] + [R-Sh-G-S] )

RP acting as a Substrate for an Enzyme in EGFR_Signaling_pathway Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
enz4 / r4	EGFR_Signaling_ pathway Accession No. : 52	EGFR Pathway No. : 223	0.05	450	4	Classical Michaelis-Menten V = Etot.S.Kcat/Km+S	Substrate RP Product R2
Taken from Kholodenko BN et al J Biol Chem. (1999) 274(42):30169-81.

RP acting as a Substrate in a reaction in EGFR_Signaling_pathway Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	r5	EGFR_Signaling_ pathway Accession No. : 52	EGFR Pathway No. : 223	60 (uM^-1 s^-1)	0.2 (s^-1)	Kd(bf) = 0.0033(uM)	-	Substrate PLCr RP Product R-PL
	Taken from Kholodenko BN et al J Biol Chem. (1999) 274(42):30169-81.
2	r13	EGFR_Signaling_ pathway Accession No. : 52	EGFR Pathway No. : 223	90 (uM^-1 s^-1)	0.6 (s^-1)	Kd(bf) = 0.0067(uM)	-	Substrate RP Shc Product R-Sh
	Taken from Kholodenko BN et al J Biol Chem. (1999) 274(42):30169-81.
3	r9	EGFR_Signaling_ pathway Accession No. : 52	EGFR Pathway No. : 223	3 (uM^-1 s^-1)	0.05 (s^-1)	Kd(bf) = 0.0167(uM)	-	Substrate Grb RP Product R-G
	Taken from Kholodenko BN et al J Biol Chem. (1999) 274(42):30169-81.

RP acting as a Product in a reaction in EGFR_Signaling_pathway Network

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	r3	EGFR_Signaling_ pathway Accession No. : 52	EGFR Pathway No. : 223	1 (s^-1)	0.01 (s^-1)	Keq = 0.01(uM)	0.99sec	Substrate R2 Product RP
	Taken from Kholodenko BN et al J Biol Chem. (1999) 274(42):30169-81.
2	r7	EGFR_Signaling_ pathway Accession No. : 52	EGFR Pathway No. : 223	0.3 (s^-1)	6 (uM^-1 s^-1)	Kd(cb) = 20(uM)	-	Substrate R-PLP Product PLCrP RP
	Taken from Kholodenko BN et al J Biol Chem. (1999) 274(42):30169-81.
3	r15	EGFR_Signaling_ pathway Accession No. : 52	EGFR Pathway No. : 223	0.3 (s^-1)	0.9 (uM^-1 s^-1)	Kd(cb) = 3(uM)	-	Substrate R-ShP Product RP ShP
	Taken from Kholodenko BN et al J Biol Chem. (1999) 274(42):30169-81.
4	r18	EGFR_Signaling_ pathway Accession No. : 52	EGFR Pathway No. : 223	0.3 (s^-1)	0.9 (uM^-1 s^-1)	Kd(cb) = 3(uM)	-	Substrate R-Sh-G Product RP Sh-G
	Taken from Kholodenko BN et al J Biol Chem. (1999) 274(42):30169-81.
5	r20	EGFR_Signaling_ pathway Accession No. : 52	EGFR Pathway No. : 223	0.12 (s^-1)	0.24 (uM^-1 s^-1)	Kd(cb) = 2(uM)	-	Substrate R-Sh-G-S Product RP Sh-G-S
	Taken from Kholodenko BN et al J Biol Chem. (1999) 274(42):30169-81.
6	r11	EGFR_Signaling_ pathway Accession No. : 52	EGFR Pathway No. : 223	0.03 (s^-1)	4.5 (uM^-1 s^-1)	Kd(cb) = 150(uM)	-	Substrate R-G-S Product G-S RP
	Taken from Kholodenko BN et al J Biol Chem. (1999) 274(42):30169-81.

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