| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents |
1 | Autophosphorylat ion | Chemotaxis Accession No. : 55 | Chemotaxis Pathway No. : 226 | 0.001 (s^-1) | 0 (s^-1) | - | - | Substrate CheA
Product CheAp
|
| Autophosphorylation of CheA by ATP Kf = 0.001 /sec Kb = 0 /sec Bray et al 1993, Mol.Biol.Cell 4: 469-482 Table 2 pp.475 Reaction Scheme 1 |
2 | Autophosphorylat ion[1] | Chemotaxis Accession No. : 55 | Chemotaxis Pathway No. : 226 | 0.0757 (uM^-1 s^-1) | 0 (s^-1) | - | - | Substrate CheA TWA
Product CheAp
|
| TWA stimulated autophosphorylation of CheA TWA is complex of Tar, CheW and CheA Kf = 7.57*10e+04 /sec/M = 0.0757 /sec/uM As per Reaction 9 in 1REACT.BCT provided by Matthew Levin Kf = 5.9*10e+4 /sec/M = 0.059 /sec/uM Kb = 0 /sec Bray et al 1993, Mol.Biol.Cell 4: 469-482 Table 2 pp.475 Reaction Scheme 2 |
3 | Autophosphorylat ion[3] | Chemotaxis Accession No. : 55 | Chemotaxis Pathway No. : 226 | 1 (uM^-1 s^-1) | 0 (s^-1) | - | - | Substrate CheA TnWA
Product CheAp
|
| Aspartate bound Tar and CheW complex TaW = 0 M As per Signal 7 in 1SIG_B.BCT Cell volume = 1.41e-15 L Table 1 pp.474 Bray et al 1993, Mol.Biol.Cell 4: 469-482 |
4 | Binding[2] | Chemotaxis Accession No. : 55 | Chemotaxis Pathway No. : 226 | 0.4 (uM^-1 s^-1) | 1 (s^-1) | Kd(bf) = 2.5(uM) | - | Substrate CheA TW
Product TWA
|
| MYpYpYpYp = 1.51e-09 M = 1.51e-3 uM As per Signal 27 in 1SIG_B.BCT provided by Matthew Levin Cell volume = 1.41e-15 L Table 1 pp.474 Bray et al 1993, Mol.Biol.Cell 4: 469-482 |
5 | Binding[3] | Chemotaxis Accession No. : 55 | Chemotaxis Pathway No. : 226 | 0.05 (uM^-1 s^-1) | 1 (s^-1) | Kd(bf) = 19.9999(uM) | - | Substrate CheA Tar
Product TA
|
| Tar-Asp-CheA complex TaA = 0 M As per Signal 10 in 1SIG_B.BCT provided by Matthew Levin Cell volume = 1.41e-15 L Table 1 pp.474 Bray et al 1993, Mol.Biol.Cell 4: 469-482 |
6 | Binding[4] | Chemotaxis Accession No. : 55 | Chemotaxis Pathway No. : 226 | 0.05 (uM^-1 s^-1) | 1 (s^-1) | Kd(bf) = 19.9999(uM) | - | Substrate CheA Tn
Product TnA
|
| Bias calculated as per formula stated on pp.471 Bias = [M + MYp] / [M + MYp + MYpYp + MYpYpYp + MYpYpYpYp] Bray et al 1993, Mol.Biol.Cell 4: 469-482 Initialy set to 0.7 |
7 | Binding[5] | Chemotaxis Accession No. : 55 | Chemotaxis Pathway No. : 226 | 0.4 (uM^-1 s^-1) | 1 (s^-1) | Kd(bf) = 2.5(uM) | - | Substrate CheA TnW
Product TnWA
|
| MYp = 9.53*e-10 M = 9.53*e-4 uM As per Signal 24 in 1SIG_B.BCT provided by Matthew Levin Cell volume = 1.41e-15 L Table 1 pp.474 Bray et al 1993, Mol.Biol.Cell 4: 469-482 |
8 | Binding[6] | Chemotaxis Accession No. : 55 | Chemotaxis Pathway No. : 226 | 0.4 (uM^-1 s^-1) | 1 (s^-1) | Kd(bf) = 2.5(uM) | - | Substrate CheA TaW
Product TaWA
|
| Motor = 8.5 molecules per cell As per Enzyme entry 7 in 1ENZ.BCT provided by Matthew Levin Motor = 6.73e-9 M = 6.73e-3 uM As per Signal 23 in 1SIG_B.BCT provided by Matthew Levin Cell volume = 1.41e-15 L Table 1 pp.474 Bray et al 1993, Mol.Biol.Cell 4: 469-482 |
9 | Binding[7] | Chemotaxis Accession No. : 55 | Chemotaxis Pathway No. : 226 | 0.05 (uM^-1 s^-1) | 1 (s^-1) | Kd(bf) = 19.9999(uM) | - | Substrate CheA TA
Product TaA
|
| Binding of Tar-Aspartate and CheA Kf = 0.05 /sec/uM as per Reaction 3 in 1REACT.BCT Kf = 1e+04 /sec/M = 0.01 /sec/uM Kb = 1 /sec Bray et al 1993, Mol.Biol.Cell 4: 469-482 Table 3 pp.476 Reaction Scheme 4 Footnote states that ligands do not have any effect on the formation of complexes so same rates are used for Aspartate or Ni associated Tar complexes. |
10 | Complexing[10] | Chemotaxis Accession No. : 55 | Chemotaxis Pathway No. : 226 | 0.05 (uM^-1 s^-1) | 1 (s^-1) | Kd(bf) = 19.9999(uM) | - | Substrate CheA CheW
Product WA
|