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Molecule Parameter List for CheA

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
CheA participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences10000102

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
Chemotaxis55Pathway
Chemotaxis 
All parameters used are from the .BCT files for BCT1.1 provided by Matthew Levin from the Computational Biology Group in the Department of Zoology at the University of Cambridge.Bias does not reach 0.7, there is a lag in the response of bias to the 5 sec 1 uM Aspartate pulse as shown by Bray et al. Mol.Biol.Cell (1993) 4(5): 469-482. The June 2003 version of the BCT program is BCT4.3 and is available at the computational biology site of the Zoology department at Cambridge University.

CheA acting as a Molecule in  
Chemotaxis Network
NameAccession NamePathway NameInitial Conc.
(uM)
Volume
(fL)
Buffered
CheAChemotaxis
Accession No. : 55
Chemotaxis
Pathway No. : 226
1.131.41No
CheA = 1.13e-06 M = 1.13 uM As per Signal in 1SIG_B.BCT provided by Matthew Levin CheA = 4250 molecules per cell As per Enzyme entry 4 in 1ENZ.BCT provided by Matthew Levin Cell volume = 1.41e-15 L Table 1 pp.474 Bray et al 1993, Mol.Biol.Cell 4: 469-482

CheA acting as a Substrate in a reaction in  
Chemotaxis Network
Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
 NameAccession NamePathway NameKfKbKdtauReagents
1
  • Autophosphorylat
    ion
  • Chemotaxis
    Accession No. : 55
    Chemotaxis
    Pathway No. : 226
    0.001
    (s^-1)
    0
    (s^-1)
    --Substrate
    CheA

    Product
    CheAp
      Autophosphorylation of CheA by ATP Kf = 0.001 /sec Kb = 0 /sec Bray et al 1993, Mol.Biol.Cell 4: 469-482 Table 2 pp.475 Reaction Scheme 1
    2
  • Autophosphorylat
    ion[1]
  • Chemotaxis
    Accession No. : 55
    Chemotaxis
    Pathway No. : 226
    0.0757
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    CheA
    TWA

    Product
    CheAp
      TWA stimulated autophosphorylation of CheA TWA is complex of Tar, CheW and CheA Kf = 7.57*10e+04 /sec/M = 0.0757 /sec/uM As per Reaction 9 in 1REACT.BCT provided by Matthew Levin Kf = 5.9*10e+4 /sec/M = 0.059 /sec/uM Kb = 0 /sec Bray et al 1993, Mol.Biol.Cell 4: 469-482 Table 2 pp.475 Reaction Scheme 2
    3
  • Autophosphorylat
    ion[3]
  • Chemotaxis
    Accession No. : 55
    Chemotaxis
    Pathway No. : 226
    1
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    CheA
    TnWA

    Product
    CheAp
      Aspartate bound Tar and CheW complex TaW = 0 M As per Signal 7 in 1SIG_B.BCT Cell volume = 1.41e-15 L Table 1 pp.474 Bray et al 1993, Mol.Biol.Cell 4: 469-482
    4Binding[2]Chemotaxis
    Accession No. : 55
    Chemotaxis
    Pathway No. : 226
    0.4
    (uM^-1 s^-1)
    1
    (s^-1)
    Kd(bf) = 2.5(uM)-Substrate
    CheA
    TW

    Product
    TWA
      MYpYpYpYp = 1.51e-09 M = 1.51e-3 uM As per Signal 27 in 1SIG_B.BCT provided by Matthew Levin Cell volume = 1.41e-15 L Table 1 pp.474 Bray et al 1993, Mol.Biol.Cell 4: 469-482
    5Binding[3]Chemotaxis
    Accession No. : 55
    Chemotaxis
    Pathway No. : 226
    0.05
    (uM^-1 s^-1)
    1
    (s^-1)
    Kd(bf) = 19.9999(uM)-Substrate
    CheA
    Tar

    Product
    TA
      Tar-Asp-CheA complex TaA = 0 M As per Signal 10 in 1SIG_B.BCT provided by Matthew Levin Cell volume = 1.41e-15 L Table 1 pp.474 Bray et al 1993, Mol.Biol.Cell 4: 469-482
    6Binding[4]Chemotaxis
    Accession No. : 55
    Chemotaxis
    Pathway No. : 226
    0.05
    (uM^-1 s^-1)
    1
    (s^-1)
    Kd(bf) = 19.9999(uM)-Substrate
    CheA
    Tn

    Product
    TnA
      Bias calculated as per formula stated on pp.471 Bias = [M + MYp] / [M + MYp + MYpYp + MYpYpYp + MYpYpYpYp] Bray et al 1993, Mol.Biol.Cell 4: 469-482 Initialy set to 0.7
    7Binding[5]Chemotaxis
    Accession No. : 55
    Chemotaxis
    Pathway No. : 226
    0.4
    (uM^-1 s^-1)
    1
    (s^-1)
    Kd(bf) = 2.5(uM)-Substrate
    CheA
    TnW

    Product
    TnWA
      MYp = 9.53*e-10 M = 9.53*e-4 uM As per Signal 24 in 1SIG_B.BCT provided by Matthew Levin Cell volume = 1.41e-15 L Table 1 pp.474 Bray et al 1993, Mol.Biol.Cell 4: 469-482
    8Binding[6]Chemotaxis
    Accession No. : 55
    Chemotaxis
    Pathway No. : 226
    0.4
    (uM^-1 s^-1)
    1
    (s^-1)
    Kd(bf) = 2.5(uM)-Substrate
    CheA
    TaW

    Product
    TaWA
      Motor = 8.5 molecules per cell As per Enzyme entry 7 in 1ENZ.BCT provided by Matthew Levin Motor = 6.73e-9 M = 6.73e-3 uM As per Signal 23 in 1SIG_B.BCT provided by Matthew Levin Cell volume = 1.41e-15 L Table 1 pp.474 Bray et al 1993, Mol.Biol.Cell 4: 469-482
    9Binding[7]Chemotaxis
    Accession No. : 55
    Chemotaxis
    Pathway No. : 226
    0.05
    (uM^-1 s^-1)
    1
    (s^-1)
    Kd(bf) = 19.9999(uM)-Substrate
    CheA
    TA

    Product
    TaA
      Binding of Tar-Aspartate and CheA Kf = 0.05 /sec/uM as per Reaction 3 in 1REACT.BCT Kf = 1e+04 /sec/M = 0.01 /sec/uM Kb = 1 /sec Bray et al 1993, Mol.Biol.Cell 4: 469-482 Table 3 pp.476 Reaction Scheme 4 Footnote states that ligands do not have any effect on the formation of complexes so same rates are used for Aspartate or Ni associated Tar complexes.
    10Complexing[10]Chemotaxis
    Accession No. : 55
    Chemotaxis
    Pathway No. : 226
    0.05
    (uM^-1 s^-1)
    1
    (s^-1)
    Kd(bf) = 19.9999(uM)-Substrate
    CheA
    CheW

    Product
    WA

    CheA acting as a Product in a reaction in  
    Chemotaxis Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1PhosphotransferChemotaxis
    Accession No. : 55
    Chemotaxis
    Pathway No. : 226
    1
    (uM^-1 s^-1)
    0
    (uM^-1 s^-1)
    --Substrate
    CheAp
    CheB

    Product
    CheA
    CheBp
      Phosphotransfer from CheAp to CheB Kf = 1 * 10e6 /sec/M = 1 /sec/uM Kb = 0 /sec/M Bray et al 1993, Mol.Biol.Cell 4: 469-482 Table 2 pp.475 Reaction Scheme 9
    2
  • Phosphotransfer[
    1]
  • Chemotaxis
    Accession No. : 55
    Chemotaxis
    Pathway No. : 226
    0.27
    (uM^-1 s^-1)
    0
    (uM^-1 s^-1)
    --Substrate
    CheAp
    CheY

    Product
    CheA
    CheYp
      Phosphotransfer from CheAp to CheY Kf = 2 * 10e+05 /sec/M = 0.2 /sec/uM Kb = 0 /sec/M Bray et al 1993, Mol.Biol.Cell 4: 469-482 Table 2 pp.475 Reaction Scheme 5



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