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Molecule Parameter List for CheW

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
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Statistics

*CheW* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	0	0	7	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Chemotaxis	55	Pathway	Chemotaxis
All parameters used are from the .BCT files for BCT1.1 provided by Matthew Levin from the Computational Biology Group in the Department of Zoology at the University of Cambridge.Bias does not reach 0.7, there is a lag in the response of bias to the 5 sec 1 uM Aspartate pulse as shown by Bray et al. Mol.Biol.Cell (1993) 4(5): 469-482. The June 2003 version of the BCT program is BCT4.3 and is available at the computational biology site of the Zoology department at Cambridge University.

CheW acting as a Molecule in Chemotaxis Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
CheW	Chemotaxis Accession No. : 55	Chemotaxis Pathway No. : 226	1.37	1.41	No
CheW = 1.37 uM As per Signal 5 in 1SIG_B.BCT provided by Matthew Levin CheW = 4250 molecules per cell As per Enzyme entry 3 in 1ENZ.BCT provided by Matthew Levin Cell volume = 1.41e-15 L Table 1 pp.474 Bray et al 1993, Mol.Biol.Cell 4: 469-482

CheW acting as a Substrate in a reaction in Chemotaxis Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	Complexing	Chemotaxis Accession No. : 55	Chemotaxis Pathway No. : 226	0.1 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 10.0003(uM)	-	Substrate CheW Tar Product TW
	CheYp = 1.24e-07 M = 1.24e-01 uM As per Signal 22 in 1SIG_B.BCT provided by Matthew Levin CheYp = 1e-08 M = 1e-2 uM As per Set_Yp configuration in 1CONFIG.BCT provided by Matthew Levin Cell volume = 1.41e-15 L Table 1 pp.474 Bray et al 1993, Mol.Biol.Cell 4: 469-482
2	Complexing[1]	Chemotaxis Accession No. : 55	Chemotaxis Pathway No. : 226	0.1 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 10.0003(uM)	-	Substrate CheW Tn Product TnW
	Nickel bound Tar and CheW complex TnW = 0.0 uM As per Signal 8 in 1SIG_B.BCT provided by Matthew Levin Cell volume = 1.41e-15 L Table 1 pp.474 Bray et al 1993, Mol.Biol.Cell 4: 469-482
3	Complexing[2]	Chemotaxis Accession No. : 55	Chemotaxis Pathway No. : 226	0.1 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 10.0003(uM)	-	Substrate CheW TA Product TaW
	Complex formation of aspartate bound Tar and CheW Kf = 1 * 10e+05 /sec/M = 0.1 /sec/uM Kb = 1 /sec Bray et al 1993, Mol.Biol.Cell 4: 469-482; Table 3 pp.476 Reaction Scheme 3 Footnote states that ligands do not have any effect on the formation of complexes so same rates are used for Aspartate or Ni associated Tar complexes.
4	Complexing[7]	Chemotaxis Accession No. : 55	Chemotaxis Pathway No. : 226	0.4 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 2.5(uM)	-	Substrate CheW TA Product TWA
	Denominator value to calculate bias as stated on pp.471 Bias = [M + MYp] / [M + MYp + MYpYp + MYpYpYp + MYpYpYpYp] Bray et al 1993, Mol.Biol.Cell 4: 469-482
5	Complexing[8]	Chemotaxis Accession No. : 55	Chemotaxis Pathway No. : 226	0.4 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 2.5(uM)	-	Substrate CheW TnA Product TnWA
	TnA and CheW complex formation Kf = 4*10e+05 /sec/M = 0.4 /sec/uM Kb = 1 /sec Bray et al 1993, Mol.Biol.Cell 4: 469-482 Table 3 pp.476 Reaction Scheme 7 Footnote states that ligands do not have any effect on the formation of Tar-CheW-CheA complex so same rates are used for Aspartate or Ni associated Tar complexes.
6	Complexing[9]	Chemotaxis Accession No. : 55	Chemotaxis Pathway No. : 226	0.4 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 2.5(uM)	-	Substrate CheW TaA Product TaWA
7	Complexing[10]	Chemotaxis Accession No. : 55	Chemotaxis Pathway No. : 226	0.05 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 19.9999(uM)	-	Substrate CheA CheW Product WA

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