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Molecule Parameter List for TWA

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
TWA participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1000013

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
Chemotaxis55Pathway
Chemotaxis 
All parameters used are from the .BCT files for BCT1.1 provided by Matthew Levin from the Computational Biology Group in the Department of Zoology at the University of Cambridge.Bias does not reach 0.7, there is a lag in the response of bias to the 5 sec 1 uM Aspartate pulse as shown by Bray et al. Mol.Biol.Cell (1993) 4(5): 469-482. The June 2003 version of the BCT program is BCT4.3 and is available at the computational biology site of the Zoology department at Cambridge University.

TWA acting as a Molecule in  
Chemotaxis Network
NameAccession NamePathway NameInitial Conc.
(uM)
Volume
(fL)
Buffered
TWAChemotaxis
Accession No. : 55
Chemotaxis
Pathway No. : 226
0.2121.41No
Tar-CheW-CheA complex TWA = 2.12e-07 M = 2.12e-1 uM As per Signal entry 13 in 1SIG_B.BCT provided by Matthew Levin Cell volume = 1.41e-15 L Table 1 pp.474 Bray et al 1993, Mol.Biol.Cell 4: 469-482

TWA acting as a Substrate in a reaction in  
Chemotaxis Network
Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
NameAccession NamePathway NameKfKbKdtauReagents
  • Autophosphorylat
    ion[1]
  • Chemotaxis
    Accession No. : 55
    Chemotaxis
    Pathway No. : 226
    0.0757
    (uM^-1 s^-1)
    0
    (s^-1)
    --Substrate
    CheA
    TWA

    Product
    CheAp
    TWA stimulated autophosphorylation of CheA TWA is complex of Tar, CheW and CheA Kf = 7.57*10e+04 /sec/M = 0.0757 /sec/uM As per Reaction 9 in 1REACT.BCT provided by Matthew Levin Kf = 5.9*10e+4 /sec/M = 0.059 /sec/uM Kb = 0 /sec Bray et al 1993, Mol.Biol.Cell 4: 469-482 Table 2 pp.475 Reaction Scheme 2

    TWA acting as a Product in a reaction in  
    Chemotaxis Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1Binding[2]Chemotaxis
    Accession No. : 55
    Chemotaxis
    Pathway No. : 226
    0.4
    (uM^-1 s^-1)
    1
    (s^-1)
    Kd(bf) = 2.5(uM)-Substrate
    CheA
    TW

    Product
    TWA
      MYpYpYpYp = 1.51e-09 M = 1.51e-3 uM As per Signal 27 in 1SIG_B.BCT provided by Matthew Levin Cell volume = 1.41e-15 L Table 1 pp.474 Bray et al 1993, Mol.Biol.Cell 4: 469-482
    2Complexing[7]Chemotaxis
    Accession No. : 55
    Chemotaxis
    Pathway No. : 226
    0.4
    (uM^-1 s^-1)
    1
    (s^-1)
    Kd(bf) = 2.5(uM)-Substrate
    CheW
    TA

    Product
    TWA
      Denominator value to calculate bias as stated on pp.471 Bias = [M + MYp] / [M + MYp + MYpYp + MYpYpYp + MYpYpYpYp] Bray et al 1993, Mol.Biol.Cell 4: 469-482
    3Binding[9]Chemotaxis
    Accession No. : 55
    Chemotaxis
    Pathway No. : 226
    0.4
    (uM^-1 s^-1)
    1
    (s^-1)
    Kd(bf) = 2.5(uM)-Substrate
    Tar
    WA

    Product
    TWA
      Binding of Tar and CheW-CheA complex Kd = 2 uM and therefore Kf also suggested as 0.5 /sec/uM Kf = 4e+05 /sec/M = 0.4 /sec/uM Kb = 1 /sec Bray et al 1993, Mol.Biol.Cell 4: 469-482 Table 3 pp.476 Reaction Scheme 8



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