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Molecule Parameter List for CaMKII-thr286

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*CaMKII-thr286* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	2	0	3	1	1	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
CaMKII_noPKA_ model3	62	Network	Shared_Object_CaMKII_noPKA_model3, CaMKII, CaM, PP1, PP2B, PP1_PSD
This is the model of CaMKII bistability, model 3. It exhibits bistability in CaMKII activation due to autophosphorylation at the PSD and local saturation of PP1. This version of model 3 does not include the full PKA regulatory pathway, and instead has a predefined initial amount of active PKA.

CaMKII-thr286 acting as a Molecule in CaMKII_noPKA_model3 Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
CaMKII-thr286	CaMKII_noPKA_ model3 Accession No. : 62	CaMKII Pathway No. : 258	0	0.09	No
I am not sure if we need to endow this one with a lot of enzs. It is likely to be a short-lived intermediate, since it will be phosphorylated further as soon as the CAM falls off.

CaMKII-thr286 acting as a Summed Molecule in CaMKII_noPKA_model3 Network

	Accession Nane	Pathway Name	Target	Input
1	CaMKII_noPKA_ model3 Accession No. : 62	CaMKII Pathway No. : 258	tot_autonomous_CaMKII	CaMKII-thr286 CaMKII*** basal_CaMKII_ cyt
2	CaMKII_noPKA_ model3 Accession No. : 62	Shared_Object_ CaMKII_noPKA_ model3 Pathway No. : 257	tot_CaMKII_cyt	CaMKII-CaM CaMKII-thr286-C aM CaMKII-thr286* CaMKII*** CaMK-thr305 CaMKII basal_CaMKII_ cyt

CaMKII-thr286 acting as a Substrate for an Enzyme in CaMKII_noPKA_model3 Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	PP1-active / Deph-thr286b	CaMKII_noPKA_ model3 Accession No. : 62	Shared_Object_ CaMKII_noPKA_ model3 Pathway No. : 257	5.09905	2.5	4	explicit E-S complex	Substrate CaMKII-thr286 Product CaMKII
2	tot_CaM_CaMKII / CaM_act_305	CaMKII_noPKA_ model3 Accession No. : 62	CaMKII Pathway No. : 258	226.998	6	4	explicit E-S complex	Substrate CaMKII-thr286 Product CaMKII***
	Rates from autocamtide phosphorylation, from Hanson and Schulman JBC 267:24 17216-17224 1992. Jan 1 1998: Speed up 12x to match fig 5.
3	tot_autonomous_ CaMKII / auton_305	CaMKII_noPKA_ model3 Accession No. : 62	CaMKII Pathway No. : 258	350	6	4	explicit E-S complex	Substrate CaMKII-thr286 Product CaMKII***
	See Hanson and Schulman again, for afterburst rates of phosph.

CaMKII-thr286 acting as a Product of an Enzyme in CaMKII_noPKA_model3 Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
PP1-active / Deph-thr305	CaMKII_noPKA_ model3 Accession No. : 62	Shared_Object_ CaMKII_noPKA_ model3 Pathway No. : 257	5.09905	2.5	4	explicit E-S complex	Substrate CaMKII* Product** CaMKII-thr286

CaMKII-thr286 acting as a Substrate in a reaction in CaMKII_noPKA_model3 Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
CaMK-thr286-bind -CaM	CaMKII_noPKA_ model3 Accession No. : 62	CaMKII Pathway No. : 258	1000.19 (uM^-1 s^-1)	0.1 (s^-1)	Kd(bf) = 0.0001(uM)	-	Substrate CaM-Ca4 CaMKII-thr286 Product CaMKII-thr286*-C aM
Affinity is up 1000X. Time to release is about 20 sec, so the kb is OK at 0.1 This makes Kf around 1.6666e-3

Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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