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Molecule Parameter List for Lambda-cl_operator

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*Lambda-cl_operator* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	1	0	0	1	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Repressilator	68	Network	Lambda-cl_gene, Lac_gene, TetR_gene
This is a network model termed as Repressilator in which LacI inhibit transcription of tetR which in turn inhibit transcription of Lambda phage. This model simulates the result as per the Fig 1c of the paper : Elowitz MB, Leibler S., Nature. 2000 Jan 20;403(6767):335-8.

Lambda-cl_operator acting as a Molecule in Repressilator Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
Lambda-cl_operator	Repressilator Accession No. : 68	Lambda-cl_gene Pathway No. : 295	0.0222	3	No
The lambda cl operator under normal conditions drives transcription of lambda cl Elowitz, M. et al. (2000) Nature 403:335-338

Lambda-cl_operator acting as an Enzyme in Repressilator Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
Lambda-cl_ operator / Lambda-cl_ basal_ transcription	Repressilator Accession No. : 68	Lambda-cl_gene Pathway No. : 295	0.0005556	0.5	4	Classical Michaelis-Menten V = Etot.S.Kcat/Km+S	Substrate Nucleotides Product lambda-cl_mRNA
Basal transcription of the Lambda cl gene. Rates from Box 1 Elowitz, M. et al. (2000) Nature 403:335-338

Lambda-cl_operator acting as a Substrate in a reaction in Repressilator Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
Lambda-cl_ operator_ inhibition_ reaction	Repressilator Accession No. : 68	Lambda-cl_gene Pathway No. : 295	1000.01 (uM^-1 s^-1)	224 (s^-1)	Kd(bf) = 0.224(uM)	-	Substrate Lambda-cl_ operator TetR Product Lambda-cl_ operator_1st_ bound
It signifies the first binding of the repressor with the operator Kf = 1 /nM/sec Kb = 224 /sec Box 1 Elowitz, M. et al. (2000) Nature 403:335-338

Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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