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Molecule Parameter List for TetR

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*TetR* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	0	1	3	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Repressilator	68	Network	Lambda-cl_gene, Lac_gene, TetR_gene
This is a network model termed as Repressilator in which LacI inhibit transcription of tetR which in turn inhibit transcription of Lambda phage. This model simulates the result as per the Fig 1c of the paper : Elowitz MB, Leibler S., Nature. 2000 Jan 20;403(6767):335-8.

TetR acting as a Molecule in Repressilator Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
TetR	Repressilator Accession No. : 68	TetR_gene Pathway No. : 297	0	3	No
TetR translated from tetR m-RNA Derived from Figure 1(c) stochastic version Elowitz, M. et al. (2000) Nature 403:335-338

TetR acting as a Product of an Enzyme in Repressilator Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
tetR_mRNA / tetR_ translation	Repressilator Accession No. : 68	TetR_gene Pathway No. : 297	0.000555595	0.167	4	Classical Michaelis-Menten V = Etot.S.Kcat/Km+S	Substrate Amino_Acids Product TetR
Translation of the tetR mRNA. Rates from Box 1 Elowitz, M. et al. (2000) Nature 403:335-338

TetR acting as a Substrate in a reaction in Repressilator Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	Lambda-cl_ operator_ inhibition_ reaction	Repressilator Accession No. : 68	Lambda-cl_gene Pathway No. : 295	1000.01 (uM^-1 s^-1)	224 (s^-1)	Kd(bf) = 0.224(uM)	-	Substrate Lambda-cl_ operator TetR Product Lambda-cl_ operator_1st_ bound
	It signifies the first binding of the repressor with the operator Kf = 1 /nM/sec Kb = 224 /sec Box 1 Elowitz, M. et al. (2000) Nature 403:335-338
2	Lambda-cl_ operator_ inhibition_ reaction[1]	Repressilator Accession No. : 68	Lambda-cl_gene Pathway No. : 295	1000.01 (uM^-1 s^-1)	9 (s^-1)	Kd(bf) = 0.009(uM)	-	Substrate Lambda-cl_ operator_1st_ bound TetR Product Lambda-cl_ operator_2nd_ bound
	It signifies the second binding of the repressor with the operator Kf = 1 /nM/sec Kb = 9 /sec Box 1 Elowitz, M. et al. (2000) Nature 403:335-338
3	TetR_ degradation	Repressilator Accession No. : 68	TetR_gene Pathway No. : 297	0.0167 (s^-1)	0 (s^-1)	-	-	Substrate TetR Product Degraded_TetR
	This reaction denotes the degradation of Tet R the half life of which is ten minutes. t1/2 = 0.693/k where k denotes kf Note: Rates had to be scaled by 1.4 to get appropriate levels Elowitz, M. et al. (2000) Nature 403:335-338

Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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