|
Enter a Search String | | Special character and space not allowed in the query term.
Search string should be at least 2 characters long. |
Molecule Parameter List for TetR-1 | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | Quorum_
repressilator | 69 | Network |
Shared_Object_Quorum_repressilator, TetR, LacI,
Lambda-cl, LacI_2nd_copy, LuxI | This model represents a single cell with the repressilator circuit, which is part of a population of similar cells. The repressilator circuit is a network of three proteins, i.e. LacI, TetR, Lambda-cI.
The single repressilator model is at accession number 68 in the DOQCS database.
In the current model, the LacI is coupled with LuxI which forms an Autoinducer(AI) protein, which couples to LuxR which activates LacI. The AI protein difffuses across the cell membrane so that the population of cells can interact. The lifetime ratio is the ratio between the mRNA and protein lifetimes. This ratio in different cells is chosen from a random gaussian distribution of mean 1 and standard deviation 0.05. The lifetime ratio of this example cell is 0.9837 |
TetR-1 acting as a Molecule in Quorum_repressilator Network
| Name | Accession Name | Pathway Name | Initial Conc.
(uM) | Volume
(fL) | Buffered | | TetR-1 | Quorum_
repressilator
Accession No. : 69 | TetR
Pathway No. : 299 | 0 | 3 | No | | TetR translated from tetR m-RNA Derived from Figure 1(c) stochastic version Elowitz, M. et al. (2000) Nature 403:335-338 |
TetR-1 acting as a Product of an Enzyme in Quorum_repressilator Network
Enzyme Molecule /
Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | tetR_mRNA1 /
tetR_
translation
| Quorum_
repressilator
Accession No. : 69 | TetR
Pathway No. : 299 | 0.000555595 | 0.167 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | Substrate
Amino_Acids
Product
TetR-1
| | Translation of the tetR mRNA. Rates from Box 1 Elowitz, M. et al. (2000) Nature 403:335-338 |
TetR-1 acting as a Substrate in a reaction in Quorum_repressilator Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| | Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | 1 | TetR_
degradation | Quorum_
repressilator
Accession No. : 69 | TetR
Pathway No. : 299 | 0.0057
(s^-1) | 0
(s^-1) | - | - | Substrate
TetR-1
Product
Degraded_TetR
| | | This reaction denotes the degradation of TetR, kf is chosen from a random gaussian distribution. Ref: Fig 2 Garcia and Elowitz et al. (2004) PNAS 101(30) 10955 - 60 | | 2 | Lambda-cl_
operator_
inhibition_
reaction | Quorum_
repressilator
Accession No. : 69 | Lambda-cl
Pathway No. : 301 | 1000.01
(uM^-1 s^-1) | 224
(s^-1) | Kd(bf) = 0.224(uM) | - | Substrate
Lambda-cl_
operator
TetR-1
Product
Lambda-cl_
operator_1st_
bound
| | | It signifies the first binding of the repressor with the operator Kf = 1 /nM/sec Kb = 224 /sec Box 1 Elowitz, M. et al. (2000) Nature 403:335-338 | | 3 | Lambda-cl_
operator_
inhibition_
reaction[1] | Quorum_
repressilator
Accession No. : 69 | Lambda-cl
Pathway No. : 301 | 1000.01
(uM^-1 s^-1) | 9
(s^-1) | Kd(bf) = 0.009(uM) | - | Substrate
Lambda-cl_
operator_1st_
bound
TetR-1
Product
Lambda-cl_
operator_2nd_
bound
| | | It signifies the second binding of the repressor with the operator Kf = 1 /nM/sec Kb = 9 /sec Box 1 Elowitz, M. et al. (2000) Nature 403:335-338 |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
This Copyright is applied to ensure that the contents of this database remain freely available. Please see FAQ for details. |
|
