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Molecule Parameter List for RCC1

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*RCC1* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	0	0	1	1

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
RanGTPase	70	Network	Shared_Object_RanGTPase, Nucleus, Cytoplasm
This model represents a concentration gradient of RanGTP across the nuclear envelope. This gradient is generated by distribution of regulators of RanGTPase. We have taken a log linear plot of graphs generated by GENESIS and compared with the experimental graphs.

RCC1 acting as a Molecule in RanGTPase Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
RCC1	RanGTPase Accession No. : 70	Nucleus Pathway No. : 305	0.7	12000	No
RCC1 Nuclear volume = 1.2 pL = 1.2e-15 m^3 Nuclear concentration of RCC1 = 0.7 uM Supplementary Table B Gorlich D et al (2003) EMBO J 2003, 22(5):1088-1100

RCC1 acting as a Substrate in a reaction in RanGTPase Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
RCC1_binding	RanGTPase Accession No. : 70	Nucleus Pathway No. : 305	74.0006 (uM^-1 s^-1)	55 (s^-1)	Kd(bf) = 0.7432(uM)	-	Substrate GDP.Ran-nuclear RCC1 Product RCC1-GDP.Ran
Binding of GDP-Ran complex with RCC1 Kf = 7.4e+07 /M/sec = 7.4e+01 /uM/sec Kb = 55 /sec Christian Klebe et al (1995) Biochemistry 34:12543-12552. Supplementary Table A Gorlich D et al (2003) EMBO J 22(5):1088-1100

RCC1 acting as a Product in a reaction in RanGTPase Network

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
GTP.Ran_release	RanGTPase Accession No. : 70	Nucleus Pathway No. : 305	55 (s^-1)	99.9994 (uM^-1 s^-1)	Kd(cb) = 1.8182(uM)	-	Substrate RCC1-RanGTP Product GTP.Ran-nuclear RCC1
Release of GTP.Ran from GTP.Ran-RCC1 complex Kf = 55 /sec Kb = 1.0e+08 /M/sec = 1.0e+02 /uM/sec Christian Klebe et al (1995) Biochemistry 34:12543-12552. Supplementary Table A Gorlich D et al (2003) EMBO J 22(5):1088-1100

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